About Enhancer
| Enhancer ID: | E_01_399 | |
| Enhancer symbol: | ||
| Species: | Human | |
| Position : | chr11:5264845-5266345   |
|
| Biosample name: | K-562 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2kb | |
| Pubmed ID: | 17283048 |
| Enhancer experiment: | FISH,Transfection | |
| Enhancer experiment description: | Minichromosomes, maintained at stable, moderate copy num_x0002_bers in K562 cells, contained the strong HS2 enhancer of the -globin LCR and the ε-globin gene in the form of a 5.2-kb minilocus in which the enhancer and gene reside about 2.5 kb apart. |
About Target gene
| Target gene : | HBE1(HBE) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP,PCR,FISH | |
| Target gene experiment description: | In K562 cells, three copies of chromosome 11, which is the loca_x0002_tion of the _x0002_-globin locus, were detected in most nuclei by hybridizing a DNA probe corresponding to the centromeric _x0008_-satellite repeat region. RNA FISH performed on the same slides revealed that only two chromosomes actively transcribe ε-globin. |
About TF
| TF name : | NFE2(NF-E2,p45) | |
| TF experiment: | ChIP | |
| TF experiment description: | Chromatin was prepared from K562 cells carrying NF-E2 mutant minichromosomes, and ChIP was car_x0002_ried out as described for panel B.Within HS2, Pol II was maximal at the core NF-E2 sites, where it is recruited by transcription activa_x0002_tors, but levels above background were detected 5_x0006_ of the core and between HS2 and ε-globin. When the NF-E2 motifs in HS2 were eliminated, Pol II was almost completely lost from the gene, consistent with the loss of HS2 enhancer activity and loss of gene transcription. |
About Function
| Enhancer function : | ε-Globin Intergenic Transcription and Histone Acetylation Dependent on an Enhancer. |
| Enhancer function experiment: | FISH,ChIP,PCR |
| Enhancer function experiment description: |
To ascertain whether this is the case,we employed DNA/RNA FISH.Here, we studied the establishment and function of histone acetylation and transcription in noncoding sequences by using a model locus linking the ε-globin HS2 enhancer and the embryonic ε-globin gene in chromatin. An intact HS2 enhancer that recruits RNA Pol II is required for intergenic transcription and histone H3 acetylation and K4 methylation between the enhancer and target gene. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
