About Enhancer
| Enhancer ID: | E_01_384 | |
| Enhancer symbol: | ||
| Species: | Human | |
| Position : | chr6:36647237-36649237   |
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| Biosample name: | MCF-7,A549,HeLa,293T | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Tumour | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | Intron | |
| Pubmed ID: | 26745862 |
| Enhancer experiment: | ChIP,qPCR | |
| Enhancer experiment description: | Our ChIP experiments showed that prior to activation p53 was exclusively found at the upstream enhancer that harbors a high-affinity, near-consen_x0002_sus binding site for p53 (Fig 5A). Occupancy of this site increased incrementally after 1.5 and 4 hours of CDK9 inhibition (Fig 5B). In contrast to this pre-loaded "primary" enhancer, treat_x0002_ment with 067 was followed by de novo recruitment of p53 to additional, secondary binding sites, most notably to an intronic region that maps to position +4 kb downstream of the p21 TSS and contains another near-consensus p53 motif (Fig 5A). After four hours of treatment,p53 occupancy of this site reached similar levels as found at the upstream enhancer (Fig 5B). |
About Target gene
| Target gene : | CDKN1A(CAP20,CDKN1,CIP1,MDA-6,P21,SDI1,WAF1,p21CIP1) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP-qPCR | |
| Target gene experiment description: | Our ChIP experiments showed that prior to activation p53 was exclusively found at the upstream enhancer that harbors a high-affinity, near-consen_x0002_sus binding site for p53 (Fig 5A). Occupancy of this site increased incrementally after 1.5 and 4 hours of CDK9 inhibition (Fig 5B). In contrast to this pre-loaded "primary" enhancer, treat_x0002_ment with 067 was followed by de novo recruitment of p53 to additional, secondary binding sites, most notably to an intronic region that maps to position +4 kb downstream of the p21 TSS and contains another near-consensus p53 motif (Fig 5A). After four hours of treatment,p53 occupancy of this site reached similar levels as found at the upstream enhancer (Fig 5B). |
About TF
| TF name : | TP53(BCC7,BMFS5,LFS1,P53,TRP53) | |
| TF experiment: | qPCR | |
| TF experiment description: | MCF7 cells, which harbour functional wild-type p53, were treated for 20 minutes to 4 hours with 10 μM 067 and both nascent unspliced precursor (pre) and mature spliced mRNA was analyzed by quantitative PCR (qPCR). Inhibitor treatment led to significant(2.5-fold) repression of nascent RNA synthesis of the p53 target gene p21 within 20 minutes (Fig1A). |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
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About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
