Enhancer ID: | E_01_380 | |
Enhancer symbol: | -- | |
Species: | Human | |
Position : | chr3:108015219-108015290 |
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Biosample name: | 293T | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | <2KB | |
Pubmed ID: | 26581162 |
Enhancer experiment: | Luciferase Reporter Assay,Transient Transfection Assay,RNA-seq,PCR | |
Enhancer experiment description: | In this study, we identify a targeted hotspot for CRISPR acti_x0002_vation within the long terminal repeat (LTR) enhancer region,at the junction between two nuclear factor (NF)-κB transcrip_x0002_tion factor–binding sites.Twenty-three possible NGG Streptococcus pyogenes(Sp) Cas9 photospacer adjacent motif (PAM) sites for targeting of sgRNAs were identified in the U3 region of the LTR, upstream of the HIV transcriptional start site(TSS) (−450 to 0 bp; HIV genome HXB2) within the enhancer and modulatory region of the promoter. |
Target gene : | HHLA2(B7-H5,B7-H7,B7H7,B7y) | |
Strong evidence: | CRISPR/Cas9 | |
Less strong evidence: | Luciferase Reporter Assay | |
Target gene experiment description: | All sgRNAs were named according to the 3′ adjacent sense strand nucleotide cleavage site catalyzed by nuclease-active Cas9 (Figure 1a) and were screened together with dCas9-VP64 or dCas9-VP160 for their activation properties by transient transfection with the reporter NL4-3.Luc.R_x005f_x0002_E-,a full-length HIV molec_x0002_ular clone where luciferase is driven by the viral LTR. |
TF name : | NFKB1(CVID12,EBP-1,KBF1,NF-kB,NF-kB1,NF-kappa-B1,NF-kappaB,NFKB-p105,NFKB-p50,NFkappaB,p105,p50) | |
TF experiment: | CRISPR/Cas9 | |
TF experiment description: | Since sg362F fully occupies the sequence across both NF-κB–binding sites, we next determined if CRISPR activation via the sg362F target site was coupled to NF-κB function. these data strongly support a conclusion where sg362F/dCas9-VP64 functions independently of NF-κB as a stand-alone transactivator of LTR activation. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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