About Enhancer
| Enhancer ID: | E_01_363 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr7:116374406-116376406   |
|
| Biosample name: | COLO 829 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Melanoma | |
| DO: | DOID:1909 | |
| Mesh: | D008545 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 24443471 |
| Enhancer experiment: | 3C,ChIP | |
| Enhancer experiment description: | Chromosome conformation capture (3C)was performed in COLO829 cells in the presence or absence of PLX4032 using the MET +63-kb enhancer as an anchor region. We observed that BRAF inhibition strikingly increased the interaction between the MET +63-kb enhancer and the MET TSS, demonstrating that this lineage-specific enhancer undergoes inducible chromatin looping in response to oncogene withdrawal in melanoma cells. |
About Target gene
| Target gene : | MET(AUTS9,DFNB97,HGFR,RCCP2,c-Met) | |
| Strong evidence: | 3C | |
| Less strong evidence: | ChIP | |
| Target gene experiment description: | Chromosome conformation capture (3C)was performed in COLO829 cells in the presence or absence of PLX4032 using the MET +63-kb Enhancer as an anchor region.We observed that BRAF inhibition strikingly increased the interaction between the MET +63-kb Enhancer and the MET TSS. |
About TF
| TF name : | MITF(CMM8,COMMAD,MI,WS2,WS2A,bHLHe32) | |
| TF experiment: | ChIP-seq,3C | |
| TF experiment description: | To test the necessity of MITF for inducible chromatin looping between the MET +63-kb Enhancer and MET TSS in response to BRAF inhibition, 3C was performed in COLO829 cells in the presence or absence of PLX4032 and MITF depletion. |
About Function
| Enhancer function : | This enhancer displays inducible chromatin looping with the MET promoter to up-regulate MET expression upon BRAF inhibition. |
| Enhancer function experiment: | ChIP,3C |
| Enhancer function experiment description: |
ChIP and 3C analysis were performed on wild-type or Enhancer-edited cells treated with PLX4032.A significant decrease in both H3K27 acetylation,a histone modification associated with active Enhancer state,and Enhancer-promoter chromatin looping was observed in Enhancer-edited cells. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
