About Enhancer
| Enhancer ID: | E_01_357 | |
| Enhancer symbol: | RET Enhancer | |
| Species: | Human | |
| Position : | chr10:43569045-43569456   |
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| Biosample name: | SK-N-BE | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 20952403 |
| Enhancer experiment: | ChIP | |
| Enhancer experiment description: | We then performed chromatin immunoprecipitation (ChIP) experiments to verify the presence of RARa, in RA-stimulated and unstimulated cells, at three different regions of RET locus:the enhancer region located about 3.4-kb upstream from the TSS, containing functional SOX10 and Pax3 binding sites and displaying a putative complete site for RARa binding; |
About Target gene
| Target gene : | RET(CDHF12,CDHR16,HSCR1,MEN2A,MEN2B,MTC1,PTC-ELE1) | |
| Strong evidence: | -- | |
| Less strong evidence: | Luciferase Reporter Assay,qRT-PCR | |
| Target gene experiment description: | The general structure of human RET gene and the main regulatory elements have been partially investigated and it has been reported that a conserved Enhancer region, located about 3000-bp upstream from a promoter region, contains binding sites for Sox10 and Pax3 transcription factors. |
About TF
| TF name : | SOX10(DOM,PCWH,WS2E,WS4,WS4C)PAX3(CDHS,HUP2,WS1,WS3) | |
| TF experiment: | ChIP | |
| TF experiment description: | We then performed chromatin immunoprecipitation (ChIP) experiments to verify the presence of RARa, in RA-stimulated and un_x0002_stimulated cells, at three different regions of RET locus (Figure 1A):(i) the Enhancer region located about 3.4-kb upstream from the TSS, containing functional SOX10 and Pax3 binding sites. |
About Function
| Enhancer function : | The primary epigenetic determinants of RA-induced RET activation differ between Enhancer and promoter regions. |
| Enhancer function experiment: | Luciferase Reporter Assay,qRT-PCR,ChIP |
| Enhancer function experiment description: |
Then we performed luciferase transcriptional assays in order to investigate whether a 3.4-kb RET gene regulatory region,located upstream from TSS and including both promoter and Enhancer,was sufficient for RA responsiveness.The results,shown in Figure 2A,demonstrated that plasmid constructs containing the 3.4-kb RET region upstream the luciferase gene were more active than empty plasmids(pGL3-basic);however, no significant increase of luciferase activity was observed when trans_x0002_fected cells were treated with RA. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
