About Enhancer
| Enhancer ID: |
|
E_01_339 |
| Enhancer symbol: |
|
gtPBREM Enhancer |
| Species: |
|
Human |
| Position : |
|
chr2:234665349-234665739
   |
| Biosample name: |
|
Hep G2 |
| Experiment class : |
|
Low throughput |
| Enhancer type: |
|
Enhancer |
| Disease: |
|
Hepatocellular Carcinoma |
| DO: |
|
DOID:684 |
| Mesh: |
|
D006528 |
| Distance from TSS: |
|
>2KB |
| Pubmed ID: |
|
30397001 |
| Enhancer experiment: |
|
Luciferase Reporter Assay,qRT-PCR |
| Enhancer experiment description: |
|
These observations suggested that a specific enhancer region, such as neighboring transcriptional factors, is required for CAR/CCAR1 interaction. |
About Target gene
| Target gene : |
|
UGT1A1(BILIQTL1,GNT1,HUG-BR1,UDPGT,UDPGT1-1,UGT1,UGT1A) |
| Strong evidence: |
|
-- |
| Less strong evidence: |
|
ChIP |
| Target gene experiment description: |
|
The phenobarbital-responsive Enhancer module of UGT1A1 (gtPBREM)-driven Luciferaseplasmid (gtPBREM-Luc.) containing a 290-bp distal Enhancer module (23570/23180) was constructed based on pGL3-promoter (Promega). |
About TF
| TF name : |
|
CCAR1(CCAR1) |
| TF experiment: |
|
ChIP |
| TF experiment description: |
|
CCAR1 regulates CAR-mediated mRNA expression (Figs. 4 and 5) and reporter activity (Figs. 2 and 3) of UGT1A1 but not CYP2B6.We speculated that CCAR1 is differentially recruited to CAR-responsive Enhancer regions. To investigate this hypothesis,we carried out ChIP experiments in HepTR/CAR cells. |
About Function
| Enhancer function : |
CCAR1 enhances transcriptional activity of CAR and acts as an Enhancer-dependent selective cofactor of CAR for UGT1A1 expression. |
| Enhancer function experiment: |
Luciferase Reporter Assay |
Enhancer function
experiment description: |
The phenobarbital-responsive Enhancer module of UGT1A1 (gtPBREM)-driven luciferase reporter plasmid (gtPBREM-Luc.) containing a 290-bp distal Enhancer module (-23570/-23180) was constructed based on pGL3-promoter (Promega). |
About SNP
| SNP ID: |
|
-- |
| SNP position: |
|
-- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
Expression of target genes for the enhancer
Enhancer associated SNPs
