About Enhancer
Enhancer ID: |
|
E_01_339 |
Enhancer symbol: |
|
gtPBREM Enhancer |
Species: |
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Human |
Position : |
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chr2:234665349-234665739
|
Biosample name: |
|
Hep G2 |
Experiment class : |
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Low throughput |
Enhancer type: |
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Enhancer |
Disease: |
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Hepatocellular Carcinoma |
DO: |
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DOID:684 |
Mesh: |
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D006528 |
Distance from TSS: |
|
>2KB |
Pubmed ID: |
|
30397001 |
Enhancer experiment: |
|
Luciferase Reporter Assay,qRT-PCR |
Enhancer experiment description: |
|
These observations suggested that a specific enhancer region, such as neighboring transcriptional factors, is required for CAR/CCAR1 interaction. |
About Target gene
Target gene : |
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UGT1A1(BILIQTL1,GNT1,HUG-BR1,UDPGT,UDPGT1-1,UGT1,UGT1A) |
Strong evidence: |
|
-- |
Less strong evidence: |
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ChIP |
Target gene experiment description: |
|
The phenobarbital-responsive Enhancer module of UGT1A1 (gtPBREM)-driven Luciferaseplasmid (gtPBREM-Luc.) containing a 290-bp distal Enhancer module (23570/23180) was constructed based on pGL3-promoter (Promega). |
About TF
TF name : |
|
CCAR1(CCAR1) |
TF experiment: |
|
ChIP |
TF experiment description: |
|
CCAR1 regulates CAR-mediated mRNA expression (Figs. 4 and 5) and reporter activity (Figs. 2 and 3) of UGT1A1 but not CYP2B6.We speculated that CCAR1 is differentially recruited to CAR-responsive Enhancer regions. To investigate this hypothesis,we carried out ChIP experiments in HepTR/CAR cells. |
About Function
Enhancer function : |
CCAR1 enhances transcriptional activity of CAR and acts as an Enhancer-dependent selective cofactor of CAR for UGT1A1 expression. |
Enhancer function experiment: |
Luciferase Reporter Assay |
Enhancer function experiment description: |
The phenobarbital-responsive Enhancer module of UGT1A1 (gtPBREM)-driven luciferase reporter plasmid (gtPBREM-Luc.) containing a 290-bp distal Enhancer module (-23570/-23180) was constructed based on pGL3-promoter (Promega). |
About SNP
SNP ID: |
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-- |
SNP position: |
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-- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
Expression of target genes for the enhancer
Enhancer associated SNPs