Enhancer ID: | E_01_329 | |
Enhancer symbol: | -- | |
Species: | Human | |
Position : | chr1:160542651-160543257 |
|
Biosample name: | HL-60,293T | |
Experiment class : | Low+High throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 30008316 |
Enhancer experiment: | ChIP-seq,ChIP-qPCR | |
Enhancer experiment description: | Notably, INTS11-gained regions were largely enriched for proximal promoters(62.2%), mirroring the known RNAPII-associated activity of the complex, and displayed robust binding of INTS13 as well. Conversely, INTS14-gained regions were largely found distal |
Target gene : | CD84(LY9B,SLAMF5,hCD84,mCD84) | |
Strong evidence: | CRISPR/Cas9,3C | |
Less strong evidence: | qRT-PCR | |
Target gene experiment description: | We performed chromosome conformation capture (3C) on the enhancer of CSF1R to infer the consequences of INTS13 depletion on genome architecture. During differentiation of HL-60 cells, we detected a robust interaction between the intronic enhancer of CSF1R and the proximal promoter, as compared to other regions within the CSF1R gene or with the promoter of neighboring genes. |
TF name : | INTS13(ASUN,C12orf11,GCT1,Mat89Bb,NET48,SPATA30)EGR1(AT225,G0S30,KROX-24,NGFI-A,TIS8,ZIF-268,ZNF225)NAB2(MADER) | |
TF experiment: | Immunoprecipitation,Colony-forming Unit (CFU) Assay | |
TF experiment description: | Immunoprecipitation of INTS13 in undifferentiated (CTRL) and differentiated (PMA) HL-60 cells confirms the interaction between INTS13 and NAB2 after PMA treatment.Colony-forming unit (CFU) assay of cord blood derived CD34+ cells infected with shRNAs against NAB2 and EGR1 shows that the number of monocytic/macrophagic colonies is significantly reduced in both NAB2- and EGR1-depleted cells when compared to control. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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