About Enhancer
| Enhancer ID: | E_01_320 | |
| Enhancer symbol: | OCT4 Intronic Enhancer | |
| Species: | Human | |
| Position : | chr6:31137269-31137697   |
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| Biosample name: | Embryonic Stem Cell | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | Intron | |
| Pubmed ID: | 29695788 |
| Enhancer experiment: | ATAC-seq,Luciferase Reporter Assay,ChIP-seq | |
| Enhancer experiment description: | In human pre-implantation blastocyst, however, neither enhancer appears open, whereas two putative enhancers appear downstream of the POU5F1 TSS. Each of these peaks contains a cluster of AP2 sites and a KLF site, indicating that they could be opened by the combinatorial activity of these transcription factors during preimplantation development. |
About Target gene
| Target gene : | POU5F1(OCT3,OCT4,OTF-3,OTF3,OTF4,Oct-3,Oct-4) | |
| Strong evidence: | CRISPR/Cas9 | |
| Less strong evidence: | -- | |
| Target gene experiment description: | We found normal expression of OCT4 and self-renewal in the primed state, but a dramatic loss of OCT4 expression accompanied by differentiation upon reversion to the naive state. This indicates a potential direct role for TFAP2C in regulating the pluripotency master-regulator OCT4 by binding to a previously unknown enhancer, which in turn is likely to be important for preimplantation OCT4 expression. |
About TF
| TF name : | TFAP2C(AP2-GAMMA,ERF1,TFAP2G,hAP-2g) | |
| TF experiment: | Luciferase Reporter Assay | |
| TF experiment description: | Luciferase activity from a pGL3 construct in which WT or mutant Intron Element 1 had been cloned, normalized to signal from a pGL3 construct with no enhancer. Results are shown from two independent experiment, except for the ΔAP2 sample, for which there are n=3 replicates from two experiments. All signals were first normalized for Renilla signal. |
About Function
| Enhancer function : | TFAP2C functions to maintain pluripotency and repress neuroectodermal differentiation during the transition from primed to naive by facilitating the opening of enhancers proximal to pluripotency factors. |
| Enhancer function experiment: | Luciferase Reporter Assay,CRISPR/Cas9 |
| Enhancer function experiment description: |
This indicates a potential direct role for TFAP2C in regulating the pluripotency master-regulator OCT4 by binding to a previously unknown Enhancer, which in turn is likely to be important for preimplantation OCT4 expression. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
