About Enhancer
| Enhancer ID: |
|
E_01_317 |
| Enhancer symbol: |
|
-- |
| Species: |
|
Human |
| Position : |
|
chr8:129046628-129096067
   |
| Biosample name: |
|
K-562 |
| Experiment class : |
|
Low+High throughput |
| Enhancer type: |
|
Super-Enhancer |
| Disease: |
|
-- |
| DO: |
|
-- |
| Mesh: |
|
-- |
| Distance from TSS: |
|
>2KB |
| Pubmed ID: |
|
29641996 |
| Enhancer experiment: |
|
4C,ChIP-seq |
| Enhancer experiment description: |
|
Among the DNA loop structures observed in these datasets, a large 2.8 Mb DNA loop was evident in all four cell types. This loop connects CTCF sites encompassing the MYC gene and qualifies as an insulated neighborhood. The DNA anchor sites of this 2.9 Mb Dƌ |
About Target gene
| Target gene : |
|
MYC(MRTLC,bHLHe39,c-Myc) |
| Strong evidence: |
|
CRISPR/Cas9 |
| Less strong evidence: |
|
ChIA-PET,ChIP-qPCR |
| Target gene experiment description: |
|
CTCF chromatin immunoprecipitation quantative polymerase chain reaction (ChIP-qPCR) showed complete loss of CTCF binding to this site in K562 and HCT-116 cells and a 60%–70% reduction in CTCF binding at this site in Jurkat and MCF8 cells. RNA analysis revŴ |
About TF
| TF name : |
|
CTCF(MRD21) |
| TF experiment: |
|
CRISPR/Cas9 |
| TF experiment description: |
|
An inducible CRISPR/Cas9 perturbation model showed reduced proliferation for these four cell types upon induction of CTCF-site deletions .These results indicate that the CTCF motif in the MYC Enhancer-docking site is necessary for CTCF binding, for high levels of MYC expression and for cellular proliferation. |
About Function
| Enhancer function : |
The CTCF site in the promoter-proximal region of MYC is important for optimal interaction with distal Enhancers and supports the idea that this CTCF site functions as an Enhancer-docking site. |
| Enhancer function experiment: |
4C,CRISPR/Cas9,ChIP-qPCR |
Enhancer function
experiment description: |
The 4C-seq data in K562 cells indicated that the MYC Enhancer docking site interacts predominantly with distal super-Enhancers located -0.3 Mb and -2 Mb downstream of the MYC gene and that the majority of these interactions were significantly reduced when the putative CTCF motif was perturbed . |
About SNP
| SNP ID: |
|
-- |
| SNP position: |
|
-- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
Expression of target genes for the enhancer
Enhancer associated SNPs
