Enhancer ID: | E_01_296 | |
Enhancer symbol: | -- | |
Species: | Human | |
Position : | chr3:184276287-184278287 |
|
Biosample name: | Colorectal Cancer | |
Experiment class : | Low+High throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 29155818 |
Enhancer experiment: | ChIP-seq,ChIP,EMSA,Luciferase Reporter Assay | |
Enhancer experiment description: | To identify candidate cis-regulatory elements targeted by FOXA factors, we performed literature search, interrogated the ECR browser for evolutionary conserved regions (ECRs), and integrated this information with the results of the FOXA1 and FOXA2 ChIP-seq analysis.Moreover, the ENCODE data base lists several FOXA1 ChIP-seq peaks at the CDH1 locus in different cell lines, especially in the second intron. Notably, at the +7.8 kb enhancer region a FOXA1 ChIP-seq signal overlapped with an ECR (Fig 5A). To find out if FOXA factors could be involved in the function of the intronic CDH1 enhancer in CRC cells, we performed ChIP analyses. |
Target gene : | EPHB3(EK2,ETK2,HEK2,TYRO6) | |
Strong evidence: | -- | |
Less strong evidence: | ChIP,Luciferase Reporter Assay | |
Target gene experiment description: | In line with this, we detected by ChIP FOXA1 occupancy at the EPHB3 −2.3 kb enhancer in LS174T and HT29 cells (Fig 5F). Furthermore, FOXA3 was found to be present at the EPHB3 enhancer at -2.3 kb in all CRC cells except for HCT116 cells.To test the importance of the FOXA binding sites for CDH1, CDX2 and EPHB3 enhancer activity, we performed luciferase reporter assays in LS174T cells. |
TF name : | FOXA1(HNF3A,TCF3A) | |
TF experiment: | EMSA,Luciferase Reporter Assay | |
TF experiment description: | EMSA to test for in vitro binding of FOXA1 and FOXA3 to the CDX2 +10.0 kb enhancer. (C) (Top) Scheme of the EPHB3 ?2.3 kb enhancer with its known transcription factor binding sites. TBE: TCF/LEF-binding element. (Bottom) EMSA to test for in vitro binding of FOXA1 and FOXA3 to the EPHB3 ?2.3 kb enhancer. (D, E, F) Luciferase reporter assay in LS174T cells with constructs covering the CDH1 (D), the CDX2 (E), and the EPHB3 (F) enhancer. Mutations of the respective FOX binding motifs are indicated by red crosses. Shown is the mean and SEM; n_x0015_3. RLA: relative luciferase activity. Statistical significance was calculated relative to the wild-type luciferase reporter constructs. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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