About Enhancer

Enhancer ID: E_01_292
Enhancer symbol: --
Species: Human
Position : chr12:47471429-47487762
Biosample name: SKmel147
Experiment class : Low+High throughput
Enhancer type: Super-Enhancer
Disease: --
DO: --
Mesh: --
Distance from TSS: >2KB
Pubmed ID:  29149598
Enhancer experiment: ATAC-seq,ChIP-seq,Luciferase Reporter Assay,Transfection,CRISPR/Cas9
Enhancer experiment description: Using Assay for Transposase-Accessible Chromatin (ATAC)-seq (Buenrostro et al., 2013) and ChIP-seq for H3K4me1 and H3K27ac (Creyghton et al., 2010), we identified ~1,400 constituent enhancers within the SEs of melanoma cells (Table S5) and found them largely devoid of open chromatin, H3K27ac, and H3K4me1 in NHMs (Figures 5F and ​and5G).To test the functionality of the predicted enhancers (Figures 6B and ​and7A), we cloned E1–E5 upstream of a minimal promoter driving luciferase expression (Prescott et al., 2015) and trans-fected these constructs into SKmel147 cells. We next used CRISPR/Cas9 editing to individually delete the genomic regions containing E2–E3 and E4–E5.

About Target gene

Target gene : AMIGO2(ALI1,AMIGO-2,DEGA)
Strong evidence: CRISPR/Cas9
Less strong evidence: Luciferase Reporter Assay
Target gene experiment description: Schematic of AMIGO2 SE showing enhancers E1–E5 (B), deleted sequences for CRISPR-Cas9 editing (C), and DNA segments used for E3 luciferase assay (D). Luciferase reporter assays performed in SKmel147 cells for AMIGO2 enhancer elements E3A–C and E3A deleted for TF motifs (E3A-Del)

About TF

TF name : FOSL2(FRA2)TEAD4(EFTR-2,RTEF1,TCF13L1,TEF-3,TEF3,TEFR-1,hRTEF-1B)
TF experiment: Immunoblot
TF experiment description: FOSL2 (left), TEAD4 (right), and AMIGO2 immunoblots of SKmel147 cells 96 hr post-transduction with shSCR or shFOSL2 (shF2 #1 and #2) and shSCR or shTEAD4+TEAD1 (shT4 #1+shT1 #1 and shT4 #2+shT1 #1). HSP90 and GAPDH were used as loading controls.

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:
--

About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs