About Enhancer
| Enhancer ID: | E_01_288 | |
| Enhancer symbol: | HGE(HER2 gene body Enhancer ) | |
| Species: | Human | |
| Position : | chr17:37879571-37880263   |
|
| Biosample name: | K-562 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Breast Cancer | |
| DO: | DOID:1612 | |
| Mesh: | D001943 | |
| Distance from TSS: | Intron | |
| Pubmed ID: | 29035388 |
| Enhancer experiment: | DNaseI-seq,ChIP-PCR,ChIP,Luciferase Reporter Assay | |
| Enhancer experiment description: | With the ENCODE Regulation Super-track Settings, DNase I hypersensitivity sites, H3K4me1 and H3K27ac were found in the previously identified intron 1 enhancer.Chromatin immunoprecipitation (ChIP)-PCR amplicons are shown. NC: sequences to which nonspecific controls were generated. |
About Target gene
| Target gene : | ERBB2(CD340,HER-2,HER-2/neu,HER2,MLN19,NEU,NGL,TKR1) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP,Luciferase Reporter Assay | |
| Target gene experiment description: | Chromatin immunoprecipitation of TFAP2C, H3K4me1 and H3K27ac were performed in SKBR3 cells and TFAP2C occupancies were confirmed at promoters 1 and 2, the intron 1 enhancer and HE.We tested the ability of the HGE to regulate the transcriptional activities of the HER2 promoters by luciferase reporter assay in 293T, SKBR3 and BT474 cells (Figure 2a). |
About TF
| TF name : | TFAP2C(AP2-GAMMA,ERF1,TFAP2G,hAP-2g) | |
| TF experiment: | CRISPR/Cas9 | |
| TF experiment description: | The transcriptional activities of these constructs were analyzed using the luciferase assay in 293T and SKBR3 cells.We next carried out genomic editing using CRISPR-Cas9 system to determine the role of the TFAP2C-binding sites at the HGE in the regulation of HER2 expression. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
