About Enhancer

Enhancer ID: E_01_267
Enhancer symbol: FBP1 Enhancer
Species: Human
Position : chr9:97396388-97401553
Biosample name: Hepatocellular Carcinoma
Experiment class : Low+High throughput
Enhancer type: Enhancer
Disease: Hepatocellular carcinoma
DO: DOID:684
Mesh: D006528
Distance from TSS: Intron
Pubmed ID:  28262837
Enhancer experiment: ChIP-seq,ChIP,RT-PCR
Enhancer experiment description: We noticed that there is a putative H3K4me1-positive enhancer region in the first intron of the FBP1 gene (Fig. 4E).Importantly, we found that at the same region there is a peak of histone H3 lysine-27 acetylation (H3K27Ac) (Fig. 4E), the level of which often correlates with transcriptional activity of the gene. We first examined whether treatment of the HDAC inhibitor NaBu affects H3K27Ac levels in this region. To this end, we erformed chromatin immunoprecipitation (ChIP) assays using H3K27Ac-specific antibody. As demonstrated in Fig. 4F, the H3K27Ac level was readily detectable in the enhancer region of FBP1 gene in both HepG2 and SK-Hep1 cell lines.However, consistent with the finding that NaBu treatment markedly increased expression of FBP1 mRNA (Fig. 2A), it also significantly increased H3K27Ac level in the FBP1 enhancer (Fig. 4F). Similar to the finding that HDAC1 and HDAC2 knockdown induced FBP1 expression, depletion of these proteins alone or together also largely increased H3K27Ac level at this locus (Fig. 4G). This data indicate that the FBP1 gene locus indeed harbors a regulatory region where H3K27Ac level can be modulated by HDAC proteins. Thus, HDAC inhibitor-induced upregulation of H3K27 acetylation in the enhancer of the FBP1 gene correlates with increased expression of FBP1 induced by HDAC inhibitors.

About Target gene

Target gene : FBP1(FBP)
Strong evidence: --
Less strong evidence: ChIP-seq,ChIP,RT-PCR
Target gene experiment description: We noticed that there is a putative H3K4me1-positive enhancer region in the first intron of the FBP1 gene (Fig. 4E).Importantly, we found that at the same region there is a peak of histone H3 lysine-27 acetylation (H3K27Ac) (Fig. 4E), the level of which often correlates with transcriptional activity of the gene. We first examined whether treatment of the HDAC inhibitor NaBu affects H3K27Ac levels in this region. To this end, we erformed chromatin immunoprecipitation (ChIP) assays using H3K27Ac-specific antibody. As demonstrated in Fig. 4F, the H3K27Ac level was readily detectable in the enhancer region of FBP1 gene in both HepG2 and SK-Hep1 cell lines.However, consistent with the finding that NaBu treatment markedly increased expression of FBP1 mRNA (Fig. 2A), it also significantly increased H3K27Ac level in the FBP1 enhancer (Fig. 4F). Similar to the finding that HDAC1 and HDAC2 knockdown induced FBP1 expression, depletion of these proteins alone or together also largely increased H3K27Ac level at this locus (Fig. 4G). This data indicate that the FBP1 gene locus indeed harbors a regulatory region where H3K27Ac level can be modulated by HDAC proteins. Thus, HDAC inhibitor-induced upregulation of H3K27 acetylation in the enhancer of the FBP1 gene correlates with increased expression of FBP1 induced by HDAC inhibitors.

About TF

TF name : --
TF experiment: --
TF experiment description: --

About Function

Enhancer function : HDAC-mediated suppression of FBP1 expression correlated with decreased histone H3K27Ac in the FBP1 enhancer.
Enhancer function experiment: ChIP,RT-PCR
Enhancer function
experiment description:
Real-time PCR analysis of DNA mmunoprecipitated by control IgG or H3K27Ac antibody from HepG2 and SK-Hep1 cells transfected with control or HDAC1- and/or HDAC2-specific siRNAs. Cells harvested for ChIP assay at 48 hours after transfectio.

About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs