About Enhancer
| Enhancer ID: | E_01_264 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr1:92921568-92937818   |
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| Biosample name: | HEL | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Super-Enhancer | |
| Disease: | Leukemia | |
| DO: | DOID:1240 | |
| Mesh: | D007938 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 28210006 |
| Enhancer experiment: | ChIP-seq,ChIP-qPCR | |
| Enhancer experiment description: | Lysine-specific demethylase 1 (LSD1, also known as KDM1A) is the first discovered histone lysine (K) demethylase and has a FAD-dependent activity to demethylase H3K4me1 and H3K4me2 but not H3K4me3.NCD25 and NCD38, small-molecule inhibitors of LSD1. In one of the LSD1 signature, the GFI1 locus, we could identify three regulatory elements defined as H3K4me2 peaks (Figure 3a). One of these elements (element 1) was thought to be a promoter because of coincidence of an H3K4me3 peak around the transcription starting sites, whereas the other elements (element 2 and element 3) were thought to be putative enhancers because of location far from the gene body and no H3K4me3 peaks. More notably, H3K27ac peaks, which are a marker of active enhancers, were elevated by NCD38 treatment in element 2. ChIP–quantitative PCR (qPCR) showed that H3K27ac level in the GFI1-SE (element 2) was elevated by twofold after 3 h of NCD38 treatment and up to sixfold after 12 h of treatment. The transcript level of GFI1 hardly increased until after 6 h of treatment but clearly increased after 12 h of treatment. |
About Target gene
| Target gene : | GFI1(GFI-1,GFI1A,SCN2,ZNF163) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP-seq,ChIP-qPCR | |
| Target gene experiment description: | In one of the LSD1 signature, the GFI1 locus, we could identify three regulatory elements defined as H3K4me2 peaks (Figure 3a). One of these elements (element 1) was thought to be a promoter because of coincidence of an H3K4me3 peak around the transcription starting sites, whereas the other elements (element 2 and element 3) were thought to be putative enhancers because of location far from the gene body and no H3K4me3 peaks. More notably, H3K27ac peaks, which are a marker of active enhancers, were elevated by NCD38 treatment in element 2. ChIP–quantitative PCR (qPCR) showed that H3K27ac level in the GFI1-SE (element 2) was elevated by twofold after 3 h of NCD38 treatment and up to sixfold after 12 h of treatment. The transcript level of GFI1 hardly increased until after 6 h of treatment but clearly increased after 12 h of treatment. |
About TF
| TF name : | -- | |
| TF experiment: | -- | |
| TF experiment description: | -- |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
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About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
