Enhancer ID: | E_01_255 | |
Enhancer symbol: | ||
Species: | Human | |
Position : | ch3:189109942-189219942 |
|
Biosample name: | Mammary Epithelial Cell MCF10A and MCF12A | |
Experiment class : | low throughput |
Enhancer type: | Enhancer | |
Disease: | Breast Cancer | |
DO: | DOID:1612 | |
Mesh: | D001943 | |
Distance from TSS: | >2KB | |
Pubmed ID: | 27980063 |
Enhancer experiment: | qPCR,ChIP | |
Enhancer experiment description: | ChIP analyses of BRD4 and RNAPII occupancy revealed that decreased BRD4 occupanc on enhancers in response to JQ1 or OTX015 treatment leads to a signiicant concomitant reduction in RNAPII occupancy on these regions (Figure 4H, I and K, L). While BRD4 occupancy was not significantly affected around the Np63 TSS, the potential enhancers displayed a substantial reduction in the occupancy of both BRD4 and RNAPII (Figure 4HandI). Most importantly, BRD4 inhibition resulted in the down regulation of the putative TP63- and GRHL3-associated eRNAs (Figure 4J and N). |
Target gene : | TP63(AIS,B(p51A),B(p51B),EEC3,KET,LMS,NBP,OFC8,RHS,SHFM4,TP53CP,TP53L,TP73L,p40,p51,p53CP,p63,p73H,p73L) | |
Strong evidence: | -- | |
Less strong evidence: | ChIP | |
Target gene experiment description: | ChIP analyses of BRD4 and RNAPII occupancy revealed that decreased BRD4 occupanc on enhancers in response to JQ1 or OTX015 treatment leads to a significant concomitant reduction in RNAPII occupancy on these regions (Figure 4H, I and K, L).Altogether, these studies demonstrate an enhancer--associated function of BRD4 in modulating the expression of TP63 and GRHL3. |
TF name : | BRD4(CAP,HUNK1,HUNKI,MCAP)EGF(HOMG4,URG)AKFOXO(FOXO) | |
TF experiment: | ChIP | |
TF experiment description: | F) and GRHL3 (G). Active transcriptional start sites and the direction of transcription are indicated by black arrows. Scale bar is denoted in kilobases. (H, I, K, L) ChIP analyses of BRD4 (H, K) and RNAPII (I, L) occupancy on TP63 (H and I) and GRHL3 (K and L) enhancer and TSS regions after JQ1/0TX015 treatment. Immunoprecipitated DNA was normalized to the input and denoted in percentage. Black dotted line denotes the background DNA precipitated by a negative control IgG. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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