About Enhancer

Enhancer ID: E_01_255
Enhancer symbol:
Species: Human
Position : ch3:189109942-189219942
Biosample name: Mammary Epithelial Cell MCF10A and MCF12A
Experiment class : low throughput
Enhancer type: Enhancer
Disease: Breast Cancer
DO: DOID:1612
Mesh: D001943
Distance from TSS: >2KB
Pubmed ID:  27980063
Enhancer experiment: qPCR,ChIP
Enhancer experiment description: ChIP analyses of BRD4 and RNAPII occupancy revealed that decreased BRD4 occupanc on enhancers in response to JQ1 or OTX015 treatment leads to a signiicant concomitant reduction in RNAPII occupancy on these regions (Figure 4H, I and K, L). While BRD4 occupancy was not significantly affected around the Np63 TSS, the potential enhancers displayed a substantial reduction in the occupancy of both BRD4 and RNAPII (Figure 4HandI). Most importantly, BRD4 inhibition resulted in the down regulation of the putative TP63- and GRHL3-associated eRNAs (Figure 4J and N).

About Target gene

Target gene : TP63(AIS,B(p51A),B(p51B),EEC3,KET,LMS,NBP,OFC8,RHS,SHFM4,TP53CP,TP53L,TP73L,p40,p51,p53CP,p63,p73H,p73L)
Strong evidence: --
Less strong evidence: ChIP
Target gene experiment description: ChIP analyses of BRD4 and RNAPII occupancy revealed that decreased BRD4 occupanc on enhancers in response to JQ1 or OTX015 treatment leads to a significant concomitant reduction in RNAPII occupancy on these regions (Figure 4H, I and K, L).Altogether, these studies demonstrate an enhancer--associated function of BRD4 in modulating the expression of TP63 and GRHL3.

About TF

TF name : BRD4(CAP,HUNK1,HUNKI,MCAP)EGF(HOMG4,URG)AKFOXO(FOXO)
TF experiment: ChIP
TF experiment description: F) and GRHL3 (G). Active transcriptional start sites and the direction of transcription are indicated by black arrows. Scale bar is denoted in kilobases. (H, I, K, L) ChIP analyses of BRD4 (H, K) and RNAPII (I, L) occupancy on TP63 (H and I) and GRHL3 (K and L) enhancer and TSS regions after JQ1/0TX015 treatment. Immunoprecipitated DNA was normalized to the input and denoted in percentage. Black dotted line denotes the background DNA precipitated by a negative control IgG.

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:
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About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs