About Enhancer

Enhancer ID: E_01_252
Enhancer symbol: IGF2 Enhancer
Species: Human
Position : chr11:2219531-2239062
Biosample name: HCC-15
Experiment class : Low+High throughput
Enhancer type: Super-Enhancer
Disease: Lung Cancer
DO: DOID:1324
Mesh: D008175
Distance from TSS: >2KB
Pubmed ID:  27869826
Enhancer experiment: ChIP-seq,Luciferase Reporter Assay
Enhancer experiment description: We next performed ChIP-Seq at the IGF2 locus, detecting an accumulation of the active chromatin mark H3K27ac at a previously identified IGF2 enhancer66 herein referred to as IGF2 cognate enhancer (Fig. 5a, Supplementary Fig. 11). An even more pronounced H3K27ac peak, however, intersected with an element previously inferred to represent a lineage-specific super-enhancer in CRC cell lines (VACO-400 and VACO-9M)46.To verify enhancer function, we performed luciferase assays revealing enhancer activity of cloned fragments of this previously inferred super-enhancer46 in the HCT116 colon cancer but not in a control (HeLa) cancer cell line (Supplementary Fig. 12 and Supplementary Table 6).

About Target gene

Target gene : IGF2(C11orf43,GRDF,IGF-II,PP9974)
Strong evidence: --
Less strong evidence: Luciferase Reporter Assay
Target gene experiment description: To verify enhancer function, we performed luciferase assays revealing enhancer activity of cloned fragments of this previously inferred super-enhancer46 in the HCT116 colon cancer but not in a control (HeLa) cancer cell line (Supplementary Fig. 12 and Supplementary Table 6).

About TF

TF name : --
TF experiment: --
TF experiment description: --

About Function

Enhancer function : this IGF2 region indeed interacts with and hence represents a candidate IGF2 Enhancer.
Enhancer function experiment: 4C-seq
Enhancer function
experiment description:
we additionally performed 4C-Seq (chromosome conformation capture sequencing63) experiments employing the putative CRE downstream of IRS4 as a viewpoint

About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs