About Enhancer
| Enhancer ID: | E_01_250 | |
| Enhancer symbol: | NgR13' Enhancer | |
| Species: | Human | |
| Position : | chr22:20224442-20228467   |
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| Biosample name: | NT2/D1 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 27602360 |
| Enhancer experiment: | Luciferase Reporter Assay,PCR | |
| Enhancer experiment description: | The presence of overlapping H3K4me1, H3K9ac, and DNase I hypersensitivity peaks suggests the presence of an enhancer in the NgR1 3 ′ region. To investigate if this region has potential enhancer activity, chr22: 20224442–20228467 was PCR amplified and cloned into a luciferase reporter gene. Substantial increases in luciferase activity were observed for both constructs in the presence of the sodium channel blocker TTX and the NMDA receptor antagonist D-AP5, which blocks neuronal activity ( fig. 4 b). |
About Target gene
| Target gene : | RTN4R(NGR,NOGOR) | |
| Strong evidence: | -- | |
| Less strong evidence: | Luciferase Reporter Assay,PCR | |
| Target gene experiment description: | The presence of overlapping H3K4me1, H3K9ac, and DNase I hypersensitivity peaks suggests the presence of an enhancer in the NgR1 3 ′ region. To investigate if this region has potential enhancer activity, chr22: 20224442–20228467 was PCR amplified and cloned into a luciferase reporter gene. Substantial increases in luciferase activity were observed for both constructs in the presence of the sodium channel blocker TTX and the NMDA receptor antagonist D-AP5, which blocks neuronal activity ( fig. 4 b). |
About TF
| TF name : | MYBL2(B-MYB,BMYB) | |
| TF experiment: | EMSA | |
| TF experiment description: | To examine if the MYB transcription factor family binds to the region with the schizophrenia risk rs701428-A allele,we performed an electrophoretic mobility shift assay (EMSA). The labeled schizophrenia risk rs701428-A allele probe, but not the labeled reference-G allele probe, formed a binding complex with c-MYB, MYBL1, and MYBL2 ( fig. 5 a, arrow). Furthermore, MYBL2 binding to the labeled rs701428-A allele probe was diminished in the presence of a 200-fold excess of unlabeled rs701428-A allele probe but not unlabeled reference-G allele probe ( fig. 5 b, arrow). |
About Function
| Enhancer function : | The NgR1 3' region, which contains rs701428, is a neuronal activity-dependent enhancer, and that the minor A allele of rs701428 is susceptible to regulation of enhancer activity by MYBL2. |
| Enhancer function experiment: | Luciferase Reporter Assay,PCR |
| Enhancer function experiment description: |
The NgR1 3 ′ Enhancer is downregulated by neuronal activation induced by KCl, and enhanced by blockade of neuronal activation by TTX and D-AP5. |
About SNP
| SNP ID: | rs701428 | |
| SNP position: | 20241019 |
| SNP experiment: | Luciferase Reporter Assay,PCR |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
