About Enhancer

Enhancer ID: E_01_239
Enhancer symbol: --
Species: Human
Position : chr1:181117806-181122451
Biosample name: HCT 116
Experiment class : Low+High throughput
Enhancer type: Enhancer
Disease: Cervical Cancer
DO: DOID:4362
Mesh: D002583
Distance from TSS: >2KB
Pubmed ID:  26754925
Enhancer experiment: ChIP-seq,Luciferase Reporter Assay,4C-seq
Enhancer experiment description: ChIP-seq analysis identified two p53 bind-ing sites 11 kb (RE1) and 46 kb (RE2) downstream of the IER5 gene (Fig. 1F).p53 binding to RE2 was strong,while binding to RE1 was relatively weak. In addition, H3K27 acetylation was detected at RE2, and was strongly increased by 5-FU treatment, showing that RE2 is an active enhancer that is highly activated upon DNA damage.We found sequences highly similar to the p53 consensus binding sequence at the center of RE2 (TRANSFAC match score; 0.94) but not in RE1 (TRANSFAC match score; 0.74, Fig. 1G). We generated DNA oligonucleotides representing RE1 and RE2, and created a version of RE2 mutated in the p53 consensus sequence (RE2 mut; sequences shown in Fig. 1G), and cloned each upstream of a luciferase reporter gene containing a minimal promoter. As shown in Fig. 1H, p53 strongly activated a reporter containing RE2, but not RE2 mut or RE1. We further performed 4C-seq analysis to identify chromatin regions that might interact with RE2. As shown in Fig.1F, we detected chromatin interaction between RE2 and RE1, and between RE2 and the IER5 gene promoter region, in both HCT116 p53 + /+ and −/− cells. The interactions between the RE2 and the IER5 promoter region seem to be stabilized by 5-FU treatment in HCT116 p53 +/+ cells but not in HCT116 p53 −/− cells. Collectively, these results show that RE2 is localized in the vicinity of the IER5 promoter when a higher order chromatin structure is formed, and identifies RE2 as the p53-responsive element of the IER5 gene.

About Target gene

Target gene : IER5(SBBI48)
Strong evidence: 4C-seq
Less strong evidence: Luciferase Reporter Assay
Target gene experiment description: We generated DNA oligonucleotides representing RE1 and RE2, and created a version of RE2 mutated in the p53 consensus sequence (RE2 mut; sequences shown in Fig. 1G), and cloned each upstream of a luciferase reporter gene containing a minimal promoter. As shown in Fig. 1H, p53 strongly activated a reporter containing RE2, but not RE2 mut or RE1. We further performed 4C-seq analysis to identify chromatin regions that might interact with RE2. As shown in Fig.1F, we detected chromatin interaction between RE2 and RE1, and between RE2 and the IER5 gene promoter region, in both HCT116 p53 + /+ and −/− cells. The interactions between the RE2 and the IER5 promoter region seem to be stabilized by 5-FU treatment in HCT116 p53 +/+ cells but not in HCT116 p53 −/− cells. Collectively, these results show that RE2 is localized in the vicinity of the IER5 promoter when a higher order chromatin structure is formed, and identifies RE2 as the p53-responsive element of the IER5 gene.

About TF

TF name : TP53(BCC7,BMFS5,LFS1,P53,TRP53)
TF experiment: Luciferase Reporter Assay
TF experiment description: ChIP-seq analyses were performed using antibodies against p53, H3K27ac, H3K4me1, H3K4me3 and phospho-RNAP II. Two p53 binding sites RE1 (11 kb downstream) and RE2 (46 kb downstream) were identified.

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:
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About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs