About Enhancer
| Enhancer ID: | E_01_221 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr20:42977441-42978040   |
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| Biosample name: | Hep G2 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Hepatocellular Carcinoma | |
| DO: | DOID:684 | |
| Mesh: | D006528 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 26232425 |
| Enhancer experiment: | Luciferase Reporter Assay | |
| Enhancer experiment description: | The HNF4a P1 promoter consists of the distal enhancer region extending around 26.5 kb fromthe transcription starting site and the proximal promoter region (Ladias et al., 1992; Parviz et al., 2003; Hatzis et al.,2006). Next, we performed cell-based Luc reporter assays using a 7 kb DNA fragment of the HNF4a P1 promoter (Hatzis and Talianidis, 2001) (Fig. 3A). RIF treatment repressed the activity of 7-kb HNF4a P1 promoter constructs by approximately 60% in ShP51 cells, but not in parental HepG2 cells. A deletion of the distal enhancer region (Δ27/26.4 kb) decreased the activity of the 7-kb construct in both parental HepG2 and ShP51 cells and abrogated the response to RIF treatment in ShP51 cells. |
About Target gene
| Target gene : | HNF4A(FRTS4,HNF4,HNF4a7,HNF4a8,HNF4a9,HNF4alpha,MODY,MODY1,NR2A1,NR2A21,TCF,TCF14) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP,Luciferase Reporter Assay | |
| Target gene experiment description: | Cell-based reporter and ChIP assays showed that PXR targeted the distal Enhancer of the HNF4a P1 promoter and stimulated dissociation of HNF3b from the distal Enhancer |
About TF
| TF name : | FOXM1(FKHL16A,FOXM1B,FOXM1C,HFH-11,HFH11,HNF-3,INS-1,MPHOSPH2,MPP-2,MPP2,PIG29,TRIDENT,FOXM1)DR1(NC2,NC2-BETA,NC2B,NCB2)HNF1A(HNF-1A,HNF1,HNF4A,IDDM20,LFB1,MODY3,TCF-1,TCF1) | |
| TF experiment: | Luciferase Reporter Assay,PCR,ChIP | |
| TF experiment description: | Twenty-four hours after transfection, cells were treated with DMSOor RIF for another 24 hours in FBS-free MEM. pRL-TK was included in all transfections as a control. Relative Luc activity was calculated by taking the activity of the cells transfected with pGL3-Basic and treated with DMSO as one. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
