About Enhancer

Enhancer ID: E_01_216
Enhancer symbol: --
Species: Human
Position : chr10:64737032-64737909
Biosample name: Mouse Embryonic Stem Cell
Experiment class : Low+High throughput
Enhancer type: Enhancer
Disease: Ewing Sarcoma
DO: DOID:3369
Mesh: D012512
Distance from TSS: >2KB
Pubmed ID:  26214589
Enhancer experiment: ChIP-seq,Luciferase Reporter Assay
Enhancer experiment description: We observed activating chromatin marks, signals for formaldehyde-assisted isolation of regulatory elements (FAIRE) and/or DNaseI hypersensitivity at five main loci: two loci corresponding to known EGR2 regulatory elements (MSE (myelinating Schwann cell enhancer) 30 and BoneE (bone enhancer) (unpublished data); Supplementary Fig. 8), one to the ADO promoter, and two to GGAA microsatellites (mSat1 and mSat2) that overlapped with EWSR1-FLI1 ChIP-Seq signals (Fig. 3a). Luciferase reporter assays indicated that BoneE and MSE had no or weak activity in Ewing sarcoma, respectively.

About Target gene

Target gene : EGR2 (AT591,CHN1,CMT1D,CMT4E,KROX20)
Strong evidence: --
Less strong evidence: ChIP-seq,Luciferase Reporter Assay
Target gene experiment description: We observed activating chromatin marks, signals for formaldehyde-assisted isolation of regulatory elements (FAIRE) and/or DNaseI hypersensitivity at five main loci: two loci corresponding to known EGR2 regulatory elements (MSE (myelinating Schwann cell enhancer) 30 and BoneE (bone enhancer) (unpublished data); Supplementary Fig. 8), one to the ADO promoter, and two to GGAA microsatellites (mSat1 and mSat2) that overlapped with EWSR1-FLI1 ChIP-Seq signals (Fig. 3a). Luciferase reporter assays indicated that BoneE and MSE had no or weak activity in Ewing sarcoma, respectively.

About TF

TF name : EWSR1(EWS,EWS-FLI1,bK984G1.4)
TF experiment: qRT-PCR
TF experiment description: ChIP efficacy was validated by qRT-PCR using a CCND1 EWSR1-FLI1 binding site.

About Function

Enhancer function : --
Enhancer function experiment: --
Enhancer function
experiment description:
--

About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs