Enhancer ID: | E_01_216 | |
Enhancer symbol: | -- | |
Species: | Human | |
Position : | chr10:64737032-64737909 |
|
Biosample name: | Mouse Embryonic Stem Cell | |
Experiment class : | Low+High throughput |
Enhancer type: | Enhancer | |
Disease: | Ewing Sarcoma | |
DO: | DOID:3369 | |
Mesh: | D012512 | |
Distance from TSS: | >2KB | |
Pubmed ID: | 26214589 |
Enhancer experiment: | ChIP-seq,Luciferase Reporter Assay | |
Enhancer experiment description: | We observed activating chromatin marks, signals for formaldehyde-assisted isolation of regulatory elements (FAIRE) and/or DNaseI hypersensitivity at five main loci: two loci corresponding to known EGR2 regulatory elements (MSE (myelinating Schwann cell enhancer) 30 and BoneE (bone enhancer) (unpublished data); Supplementary Fig. 8), one to the ADO promoter, and two to GGAA microsatellites (mSat1 and mSat2) that overlapped with EWSR1-FLI1 ChIP-Seq signals (Fig. 3a). Luciferase reporter assays indicated that BoneE and MSE had no or weak activity in Ewing sarcoma, respectively. |
Target gene : | EGR2 (AT591,CHN1,CMT1D,CMT4E,KROX20) | |
Strong evidence: | -- | |
Less strong evidence: | ChIP-seq,Luciferase Reporter Assay | |
Target gene experiment description: | We observed activating chromatin marks, signals for formaldehyde-assisted isolation of regulatory elements (FAIRE) and/or DNaseI hypersensitivity at five main loci: two loci corresponding to known EGR2 regulatory elements (MSE (myelinating Schwann cell enhancer) 30 and BoneE (bone enhancer) (unpublished data); Supplementary Fig. 8), one to the ADO promoter, and two to GGAA microsatellites (mSat1 and mSat2) that overlapped with EWSR1-FLI1 ChIP-Seq signals (Fig. 3a). Luciferase reporter assays indicated that BoneE and MSE had no or weak activity in Ewing sarcoma, respectively. |
TF name : | EWSR1(EWS,EWS-FLI1,bK984G1.4) | |
TF experiment: | qRT-PCR | |
TF experiment description: | ChIP efficacy was validated by qRT-PCR using a CCND1 EWSR1-FLI1 binding site. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
-- |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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