About Enhancer
| Enhancer ID: | E_01_214 | |
| Enhancer symbol: | PRE3 Enhancer | |
| Species: | Human | |
| Position : | chr8:81286250-81293977   |
|
| Biosample name: | Lymphoblastoid Cell Lines | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer | |
| Disease: | National Institute of Allergy and Infectious Diseases | |
| DO: | -- | |
| Mesh: | D054579 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 26211970 |
| Enhancer experiment: | ChIP-seq,Luciferase Reporter Assay,PCR,3C | |
| Enhancer experiment description: | PRE2 (A) or PRE3 (B) was cloned upstream of a PAG1 promoter driven luciferase reporter with and without the candidate causal SNPs.Our 3C studies provided evidence for allele--specific chromatin looping between PRE2 and PAG1. These results demonstrate that PRE3 acts as a transcriptional enhancer in the presence of the rs2370615:C allergy predisposing allele. |
About Target gene
| Target gene : | PAG1(CBP,PAG) | |
| Strong evidence: | 3C | |
| Less strong evidence: | Luciferase Reporter Assay | |
| Target gene experiment description: | PRE2 (A) or PRE3 (B) was cloned upstream of a PAG1 promoter driven luciferase reporter with and without the candidate causal SNPs.Our 3C studies provided evidence for allele--specific chromatin looping between PRE2 and PAG1. These results demonstrate that PRE3 acts as a transcriptional enhancer in the presence of the rs2370615:C allergy predisposing allele. |
About TF
| TF name : | NFKB1(CVID12,EBP-1,KBF1,NF-kB,NF-kB1,NF-kappa-B1,NF-kappaB,NFKB-p105,NFKB-p50,NFkappaB,p105,p50) | |
| TF experiment: | ChIP-seq | |
| TF experiment description: | Based on ENCODE ChIP-seq data for LCLs,17 two transcription factors (TFs) bind to PRE3 and overlap the putative functional variant rs2370615: the RelA (or p65[MIM: 164014]) subunit of the nuclear factor (NF-)TF,which is critical for innate and adaptive immune re_x0002_sponses,22 and the POU domain class 2 transcription factor2 (POU2F2 or Oct-2 [MIM: 164176]),which regulates B-cell-specific genes. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
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About SNP
| SNP ID: | rs7009110 | |
| SNP position: | 81291879 |
| SNP experiment: | ChIP-seq,Luciferase Reporter Assay,3C |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
