About Enhancer
| Enhancer ID: | E_01_203 | |
| Enhancer symbol: | hCNS1 Enhancer | |
| Species: | Human | |
| Position : | chr17:61927353-61928827   |
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| Biosample name: | GC-spd(ts),Hepa 1-6,293T | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 25622893 |
| Enhancer experiment: | PCR,Luciferase Reporter Assay | |
| Enhancer experiment description: | We first investigated enhancer activity of hCNS1. Because TSS of TCAM1P--I and TCAM1P--III was +401 and three TSSs of TCAM1P--II were close to +401 (Fig. 2E), we cloned about 2--kb sequence upstream of +401 as a major promoter of the TCAM1P gene and connected it to the luciferase gene (TCAM1P--Pro--luc). hCNS1 was amplified by genome PCR and cloned to upstream of the TCAM1P promoter (hCNS1--Pro--luc) or to downstream of the luciferase gene(Pro--luc--hCNS1 and Pro--luc--rev hCNS1).The activity of hCNS1--Pro--luc was significantly increased in all three cell lines compared to the construct without hCNS1 (Fig. 3A). |
About Target gene
| Target gene : | TCAM1P(TCAM1) | |
| Strong evidence: | -- | |
| Less strong evidence: | Luciferase Reporter Assay | |
| Target gene experiment description: | We also examined the luciferase activity in linearized constructs. If hCNS1 was actually a DPE, it might drive the transcription of the entire sequence in a circular plasmid, which might affect the luciferase gene expression.To prevent hCNS1 from driving the luciferase gene transcription in the sense direction, we linearized Pro--luc--hCNS1 and Pro--luc--rev hCNS1 constructs by cutting the immediate downstream sequence of hCNS1 and rev hCNS1. As a result, hCNS1 still significantly increased TCAM1P promoter activity in HEK293T cells, and the fold--increases were around 3 in both constructs compared to the linearized TCAM1P--Pro--luc construct (Fig. 3B). These data showed that hCNS1 could function as an enhancer for the TCAM1P gene. |
About TF
| TF name : | -- | |
| TF experiment: | -- | |
| TF experiment description: | -- |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
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About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
