About Enhancer
| Enhancer ID: | E_01_170 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr19:51358995-51369041   |
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| Biosample name: | LNCaP,VCaP | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Prostate Cancer | |
| DO: | DOID:10283 | |
| Mesh: | D011471 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 24778216 |
| Enhancer experiment: | Luciferase Reporter Assay,RT-PCR,ChIP,3C | |
| Enhancer experiment description: | Because the induction of eRNA is strongly correlated with the enhancer looping to target gene promoters，it is possible that the KLK3 enhancer element is able to interact with the KLK2 promoter. To address this, we used the chromosome conformation capture (3C) assay to study the spatial interaction of the KLK3/2 loci. It should be noted that the KLK2 promoter contains an ARE I that shares over 80% sequence homology with the ARE I in the KLK3 promoter. |
About Target gene
| Target gene : | KLK3(APS,KLK2A1,PSA,hK3) | |
| Strong evidence: | 3C | |
| Less strong evidence: | RT-PCR | |
| Target gene experiment description: | In fact, the transcription of KLK3 eRNA (KLK3e) was demon_x0002_strated by GRO-seq data (10) and confirmed by our reverse transcription–quantitative PCR (RT-qPCR) analyses in andro_x0002_gen-dependent LNCaP and VCaP, and androgen-independent LNCaP-abl cells (21). |
About TF
| TF name : | AR(AIS8,DHTR,HUMARA,HYSP1,KD,NR3C4,SBMA,SMAX1,TFM,AR) | |
| TF experiment: | RT-PCR | |
| TF experiment description: | The effect of siKLK3e on AR-regulated gene expression was determined by RT-qPCR.our data show that KLK3e selectively regulates the expression of AR-regulated genes that do not reside on the same chromosome,implying a transregulatory function of KLK3e on AR dependent gene expression. |
About Function
| Enhancer function : | KLK3e carries the core enhancer element derived from the androgen response element III (ARE III).KLK3e facilitates the spatial interaction of the KLK3 Enhancer and the KLK2 promoter and enhances long-distance KLK2 transcriptional activation. |
| Enhancer function experiment: | RT-PCR,3C,RIP,3C-qPCR |
| Enhancer function experiment description: |
Kallikrein-related peptidase 3 (KLK3), which codes for prostate-specific antigen (PSA), is a well-known AR-regulated gene and its upstream enhancers produce bi- directional enhancer RNAs (eRNAs), termed KLK3e. Here, we demonstrate that KLK3e facilitates the spatial interaction of the KLK3 enhancer and the KLK2 promoter and enhances long-distance KLK2 transcriptional activation. KLK3e carries the core enhancer element derived from the androgen response element III (ARE III), which is required for the interaction of AR and Mediator 1 (Med1). Furthermore, we show that KLK3e processes RNA-dependent enhancer activity depending on the integrity of core enhancer elements. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
