Enhancer ID: | E_01_164 | |
Enhancer symbol: | -- | |
Species: | Human | |
Position : | chr5:139987854-139989680 |
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Biosample name: | Peripheral Blood Monocytes | |
Experiment class : | Low+High throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 24671955 |
Enhancer experiment: | RT-PCR,ChIP,Luciferase Reporter Assay,ChIP-seq | |
Enhancer experiment description: | To extend the analysis of regulatory sites to putative enhancers, we also carried out ChIP sequencing for 2 histone marks, namely H3K4me1 and H3K27ac,that were previously associated with enhancers.30-34 H3K27 is a major substrate for the coactivators p300 and CBP and its acetylation marks active enhancers, whereas H3K4me1 is generally associated with distal regulatory elements, including poised enhancers. |
Target gene : | CD14(CD14) | |
Strong evidence: | -- | |
Less strong evidence: | RT-PCR,Luciferase Reporter Assay | |
Target gene experiment description: | On this basis, we sought an explanation for the differential ex_x0002_pression of CD14 in classical monocytes. Studies in transgenic mice by Zhang and coworkers established that a region of 80 kb surrounding the human CD14 gene is sufficient to direct its monocyte specific expression,42 whereas smaller constructs recapitulated human liver expression but failed to direct monocyte-specific expression.The genomic interval downstream of the CD14 gene contained a number of H3K27ac-marked sites specific for classical,CD14-expressing monocytes.These sites frequently overlapped with bidirectional enhancers identified using the FANTOM5 expression atlas44 as well as binding sites for PU.1 or C/EBPb in total monocytes,18 which are key factors in establishing distal regulatory sites in these cells. |
TF name : | -- | |
TF experiment: | -- | |
TF experiment description: | -- |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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