About Enhancer
| Enhancer ID: | E_01_159 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr17:60986269-60989232   |
|
| Biosample name: | HEK293FT,FLP-IN T-REX 293 CELLS | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Colorectal Cancer | |
| DO: | DOID:9256 | |
| Mesh: | D015179 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 24667606 |
| Enhancer experiment: | qRT-PCR,ChIP,Luciferase Reporter Assay | |
| Enhancer experiment description: | Here we tested whether dTALEs can be used to target genes whose expression is controlled by Wnt/β-catenin signaling.TALE DNA binding domains were engineered to recognize sequences adjacent to Wnt responsive enhancer elements (WREs) that control expression of axis inhibition protein 2 (AXIN2) and c-MYC (MYC).The TALE-SIDs repressed luciferase reporter activity, bound their genomic target sites, and repressed AXIN2 and MYC expression in HEK293 cells. We generated a novel HEK293 cell line to determine whether the TALE-SIDs could function downstream of oncogenic Wnt/β-catenin signaling. |
About Target gene
| Target gene : | AXIN2(AXIL,ODCRCS) | |
| Strong evidence: | -- | |
| Less strong evidence: | qRT-PCR,ChIP,Luciferase Reporter Assay | |
| Target gene experiment description: | The AXIN2 luciferase reporter plasmid was generated by PCR-amplifying a 787-bp fragment of the AXIN2 gene from genomic HCT116 DNA that includes the TALE binding sites. The PCR product was sub-cloned into the pGL3 basic vector as a KpnINheI fragment. To generate the pcDNA5/FRT/TO-β-cateninS45F-estrogen receptor (ER) expression plasmid, β-cateninS45F cDNA was PCR-amplified from pcDNA3-β-catenin. |
About TF
| TF name : | SID(SID) | |
| TF experiment: | ChIP,qRT-PCR | |
| TF experiment description: | The MYC luciferase transgene, which contains approximately 8.6-kb of genomic DNA that encompasses MYC, was generated stepwise with two PCR fragments amplified from a bacterial artificial chromosome (BAC) that harbors human MYC. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
-- |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
