Enhancer ID: | E_01_158 | |
Enhancer symbol: | -- | |
Species: | Human | |
Position : | chr17:60983452-60984775 |
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Biosample name: | HEK293FT,FLP-IN T-REX 293 CELLS | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | Colorectal Cancer | |
DO: | DOID:9256 | |
Mesh: | D015179 | |
Distance from TSS: | >2KB | |
Pubmed ID: | 24667606 |
Enhancer experiment: | qRT-PCR,ChIP,Luciferase Reporter Assay | |
Enhancer experiment description: | Here we tested whether dTALEs can be used to target genes whose expression is controlled by Wnt/β-catenin signaling.TALE DNA binding domains were engineered to recognize sequences adjacent to Wnt responsive enhancer elements (WREs) that control expression of axis inhibition protein 2 (AXIN2) and c-MYC (MYC).The TALE-SIDs repressed luciferase reporter activity, bound their genomic target sites, and repressed AXIN2 and MYC expression in HEK293 cells. We generated a novel HEK293 cell line to determine whether the TALE-SIDs could function downstream of oncogenic Wnt/β-catenin signaling. |
Target gene : | AXIN2(AXIL,ODCRCS) | |
Strong evidence: | -- | |
Less strong evidence: | qRT-PCR,ChIP,Luciferase Reporter Assay | |
Target gene experiment description: | The AXIN2 luciferase reporter plasmid was generated by PCR-amplifying a 787-bp fragment of the AXIN2 gene from genomic HCT116 DNA that includes the TALE binding sites. The PCR product was sub-cloned into the pGL3 basic vector as a KpnINheI fragment. To generate the pcDNA5/FRT/TO-β-cateninS45F-estrogen receptor (ER) expression plasmid, β-cateninS45F cDNA was PCR-amplified from pcDNA3-β-catenin. |
TF name : | SID(SID) | |
TF experiment: | ChIP,qRT-PCR | |
TF experiment description: | We conducted ChIP assays with FLAG antibodies in transiently transfected cells and found that both TALE1-SID and TALE2-SID displayed enriched binding to their target sites within AXIN2 relative to a control site that mapped within the 3’untranslated region (Fig. 2A, B). A qRT-PCR analysis of transcripts found that cells transfected with both TALE-SIDs contained 5-fold lower levels of AXIN2 expression relative to control. |
Enhancer function : | -- |
Enhancer function experiment: | -- |
Enhancer function experiment description: |
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SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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