About Enhancer
| Enhancer ID: | E_01_156 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr8:119677099-119681977   |
|
| Biosample name: | HT-29,HCT 116 | |
| Experiment class : | Low throughput |
| Enhancer type: | Super-Enhancer | |
| Disease: | Colorectal Cancer | |
| DO: | DOID:9256 | |
| Mesh: | D015179 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 24662484 |
| Enhancer experiment: | qRT-PCR,3C,ChIP | |
| Enhancer experiment description: | The human 8q24 gene desert contains multiple enhancers that form tissue-specific long-range chromatin loops with the MYC oncogene, but how chromatin looping at the MYC locus is regulated remains poorly understood. Here we demonstrate that a long noncoding RNA (lncRNA), CCAT1-L, is transcribed specifically in human colorectal cancers from a locus 515 kb upstream of MYC. This lncRNA plays a role in MYC transcriptional regulation and promotes long-range chromatin looping. |
About Target gene
| Target gene : | MYC(MRTLC,bHLHe39,c-Myc) | |
| Strong evidence: | 3C | |
| Less strong evidence: | qRT-PCR,ChIP | |
| Target gene experiment description: | Importantly, we found that knock_x0002_down of CCAT1-L greatly reduced the transcription of nascent MYC RNA (Figure 2C), further suggesting that CCAT1-L regulates MYC expression at the transcriptional level. Nevertheless, while the reduction on the steady_x0002_state MYC mRNA that we observed was modest, it is possible that this at least partly reflects the known com_x0002_plexity of MYC regulation. |
About TF
| TF name : | TCF4(E2-2,FECD3,ITF-2,ITF2,PTHS,SEF-2,SEF2,SEF2-1,SEF2-1A,SEF2-1B,SEF2-1D,TCF-4,bHLHb19)CTCF(MRD21) | |
| TF experiment: | ChIP-seq | |
| TF experiment description: | Analyses of the available ChIP-seq datasets [39] re_x0002_vealed that TCF4 and CTCF are highly enriched in this 8q24 region in HCT116 cells (Figure 6A). Extensive TCF4 binding in the chromatin region between MYC-515 and MYC-335 was consistent with the notion that this chromatin region is a super-Enhancer that can recruit transcriptional factors. |
About Function
| Enhancer function : | The CCAT1-L locus is located within a strong super-enhancer and is spatially close to MYC |
| Enhancer function experiment: | 3C,RT-PCR |
| Enhancer function experiment description: |
Here we demonstrate that a long noncoding RNA (lncRNA), CCAT1-L, is transcribed specifically in human colorectal cancers from a locus 515 kb upstream of MYC. This lncRNA plays a role in MYC transcriptional regulation and promotes long-range chromatin looping. Importantly, the CCAT1-L locus is located within a strong super-enhancer and is spatially close to MYC. Knockdown of CCAT1-L reduced long-range interactions between the MYC promoter and its enhancers. |
About SNP
| SNP ID: | rs6983267 | |
| SNP position: | 128413305 |
| SNP experiment: | qRT-PCR,3C |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
