About Enhancer
| Enhancer ID: | E_01_147 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr3:188892648-189232665   |
|
| Biosample name: | B-Cell Lymphoma Cell Lines | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer | |
| Disease: | Diffuse Large B-Cell Lymphoma | |
| DO: | DOID:0050745 | |
| Mesh: | D016403 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 24332044 |
| Enhancer experiment: | ChIP-seq,ChIP-qPCR | |
| Enhancer experiment description: | As predicted, BRD4 load is asymmetrically distributed throughout the genome at enhancer sites. Completely unexpected is the magnitude by which BRD4 load varies among active enhancer regions (Figure 4E). Only a small subset of BRD-loaded enhancers, 285/18330(1.6%), account for 32% of all of the BRD4 enhancer binding in the cell.The BRD4-loaded enhancers in the Ly1 DLBCL cell line are considerably larger than typical enhancer elements, resembling the super-enhancers we recently described with Richard Young. |
About Target gene
| Target gene : | BCL6(BCL5A,LAZ3,ZBTB27,ZNF51,BCL6) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP-seq | |
| Target gene experiment description: | Notably, the top two gene loci with BRD4-loaded enhancers, POU2AF1 (which encodes the OCA-B transcriptional co-activator protein) and BCL6, and additional genes with disproportionally BRD4-loaded enhancers such as PAX5 and IRF8 (Figure 4E), are essential for B-cell fate determination and germinal center formation. |
About TF
| TF name : | E2F1(E2F-1,RBAP1,RBBP3,RBP3) | |
| TF experiment: | ChIP-seq,ChIP-qPCR | |
| TF experiment description: | Then, we assessed the genome-wide localization of E2F1 and the representative BET protein, BRD4, also by ChIP-Seq using the respective antibodies. Rank-ordering of all transcriptionally active promoters based on H3K4me3 enrichment and RNA Pol II occupancy identifies pervasive binding of BRD4 and E2F1 to active promoter elements. |
About Function
| Enhancer function : | These super-Enhancers prove particularly sensitive to bromodomain inhibition,explaining the selective effect of BET inhibitors on oncogenic and lineage-specific transcriptional circuits. |
| Enhancer function experiment: | ChIP-seq,ChIP-qPCR |
| Enhancer function experiment description: |
These super-enhancers prove particularly sensitive to bromodomain inhibition, explaining the selective effect of BET inhibitors on oncogenic and lineage-specific transcriptional circuits. Functional study of genes marked by super-enhancers identifies DLBCLs dependent on OCA-B and suggests a strategy for discovering unrecognized cancer dependencies. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
