About Enhancer
| Enhancer ID: | E_01_137 | |
| Enhancer symbol: | ECR18 Enhancer | |
| Species: | Human | |
| Position : | chr19:57566145-57571006   |
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| Biosample name: | HeLa,HEK-293 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 24040411 |
| Enhancer experiment: | Luciferase Reporter Assay,PCR,3C,ChIP | |
| Enhancer experiment description: | The observed interaction is most prominent in brain, but was also detected in testis. Histone modification and DNA methylation on ECR18 show no allele bias, implying that this region is likely functional on both alleles. In vitro assays also reveal ECR18 as a potential enhancer or repressor for the promoter of Peg3. Overall, these results indicate that the promoters of several imprinted genes in the Peg3 domain interact with one evolutionarily conserved region, ECR18, and further suggest that ECR18 may play key roles in the transcription and imprinting control of the Peg3 domain as a distant regulatory element. |
About Target gene
| Target gene : | PEG3(PW1,ZKSCAN22,ZNF904,ZSCAN24) | |
| Strong evidence: | 3C | |
| Less strong evidence: | Luciferase Reporter Assay,PCR | |
| Target gene experiment description: | In the current study, the chromatin conformation capture (3C) method was utilized to detect potential interactions of these ECRs with the imprinted genes. According to the results, one region, ECR18, located 200-kb upstream of Peg3 interacts with the two promoter regions of Peg3 and Zim2. The observed interaction is most prominent in brain, but was also detected in testis. |
About TF
| TF name : | -- | |
| TF experiment: | -- | |
| TF experiment description: | -- |
About Function
| Enhancer function : | ECR18 may play key roles in the transcription and imprinting control of the Peg3 domain as a distant regulatory element. |
| Enhancer function experiment: | 3C,ChIP |
| Enhancer function experiment description: |
In the current study, we performed a series of Chromatin Conformation Capture (3C) and Chromatin Immunoprecipitation (ChIP) analyses to further confirm this prediction. Overall, the current study identifies one region, ECR18, as a key regulatory region for the transcription and imprinting of the Peg3 domain. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
