About Enhancer
| Enhancer ID: | E_01_134 | |
| Enhancer symbol: | ERE Enhancer | |
| Species: | Human | |
| Position : | chr6:161110725-161112725   |
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| Biosample name: | Hep G2,MCF-7 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Hypoplasminogenemia | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 23872150 |
| Enhancer experiment: | Luciferase Reporter Assay,ChIP,EMSA,PCR | |
| Enhancer experiment description: | An AP3-like (activator protein 3) enhancer element, located 2.4 kb upstream of PLG-TSS, is involved in PLG transcriptional regulationIn summary we demonstrated that estrogen regulates plasminogen promoter activity. The 5’-flanking region of the PLG gene con_x0002_tains several cis-acting elements that are responsive to estrogen.An enhancer located at -11.5 kb confers a dramatic estrogen re_x0002_sponse in reporter assays. |
About Target gene
| Target gene : | PLG(PLG) | |
| Strong evidence: | -- | |
| Less strong evidence: | Luciferase Reporter Assay,PCR | |
| Target gene experiment description: | In our study we could show that a construct comprising the entire upstream region, containing the AP3 re_x0002_sponse element (2.4 kb-PLG) was able to further elevate basal luciferase activity 1.7-fold above that of PLG minimal promoters.The observed increase corresponds well to previously published results [20] and the 2.4 kb-PLG construct represents the most active contiguous promoter fragment analysed in this study. Surprisingly, with E2 stimulation this effect decreased by 54% down to the activity level of the PLG core promoter,although no ERE could be identified within this region. |
About TF
| TF name : | DHII(anon-EST:Posey114,anon-WO02059370.45,CG13094,DH31,DH-II,dh31,DH31,DH[31],DH[[31],Dh[[31]],DH[[31]],Dmel\CG13094,Drm-DH[[31]],drome-DH31,Drome-DH[[31]])ERE(ERE) | |
| TF experiment: | Luciferase Reporter Assay | |
| TF experiment description: | Transcriptional effect of the DHII and/or ERE (_x0003_11.5 kb) enhancers on the heterologous crystallin promoter (cryst) driven luciferase activity in HepG2. |
About Function
| Enhancer function : | The intergenic region between both genes comprises several transcription-regulatory regions with Enhancer sequences that increase the basal activity of the PLG core promoter. |
| Enhancer function experiment: | Luciferase Reporter Assay |
| Enhancer function experiment description: |
An estrogen response element located 11.5 kb upstream of the PLG transcription start site is able to convey a dramatic estrogen-dependent elevation of PLG-minimal promoter driven reporter gene expression. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
