About Enhancer
| Enhancer ID: | E_01_122 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr9:21816751-21818751   |
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| Biosample name: | Human 2BS Diploid Fibroblast Cells | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 23443045 |
| Enhancer experiment: | ChIP-qPCR,3C,ChIP,Luciferase Reporter Assay,EMSA | |
| Enhancer experiment description: | Finally, a systematic survey of putative FOXA1 binding sites in the p16INK4a genomic region revealed an B150 kb distal element that could loop back to the promoter and potenti ate p16INK4a expression.To further investigate the possibility that this 150 kb element can function as authentic enhancer, we inserted a stretch of genomic sequence centred on this site into the luciferase vector containing the p16INK4a promoter and co-transfected these constructs with FOXA1 expression plas_x0002_mids into HeLa cells. |
About Target gene
| Target gene : | CDKN2A(ARF,CDK4I,CDKN2,CMM2,INK4,INK4A,MLM,MTS-1,MTS1,P14,P14ARF,P16,P16-INK4A,P16INK4,P16INK4A,P19,P19ARF,TP16) | |
| Strong evidence: | -- | |
| Less strong evidence: | qPCR,ChIP,Luciferase Reporter Assay | |
| Target gene experiment description: | To determine whether FOXA1 was required for p16INK4a activation in replicative exhaustion and OIS, we knocked down FOXA1 using lentiviral shRNA and examined the resultant expression change of p16INK4a. As expected, induc_x0002_tion of p16INK4a protein at late passage was impaired in 2BS cells which integrated with lentiviral shRNA for FOXA1 in middle-aged stage. |
About TF
| TF name : | FOXA1(HNF3A,TCF3A) | |
| TF experiment: | ChIP,Luciferase Reporter Assay,EMSA | |
| TF experiment description: | Of note,-2 histone positioning site contains the FOXA1’s binding motif identified through ChIP, luciferase reporter assay and EMSA as stated above. |
About Function
| Enhancer function : | An B150 kb distal element could loop back to the promoter and potentiate p16INK4a expression. DNA variant at the 150 kb enhancer in?uences FOXA1-dependent p16INK4a activation. |
| Enhancer function experiment: | Luciferase Reporter Assay,EMSA |
| Enhancer function experiment description: |
Importantly, mutation of FOXA1 binding site at promoter region completely abolished the induced luciferase activity, underscoring that the function of -150 kb Enhancer was dependent on its communication with the promoter bridged by FOXA1.DNA variant at the 150 kb enhancer in?uences FOXA1-dependent p16INK4a activation. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
