About Enhancer
| Enhancer ID: | E_01_110 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr12:8035824-8037824   |
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| Biosample name: | HEK-293,HeLa | |
| Experiment class : | Low+High throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 22645302 |
| Enhancer experiment: | ChIP-PCR,RT-PCR,3C-ChIP,ChIP-seq | |
| Enhancer experiment description: | To examine whether histone enhancers mark changes under hypoxia, we compared the HIF1 binding sites and H3K27ac under normoxia and hypoxia on a genome-wide scale. The number of total reads and uniquely mapped sequences of the ChIP-seq for histone marks are shown in Table S4 in the supplemental material. H3K27ac sites overlapped at 12,498 sites between the state of normoxia (14,679 sites) and hypoxia (16,366 sites) on a genome-wide scale.The results of the HIF1 ChIP-seq analysis provided data of hypoxic enhancer 2 (kbp -24 from the TSS) at the SLC2A3 loci. |
About Target gene
| Target gene : | SLC2A3(GLUT3) | |
| Strong evidence: | 3C | |
| Less strong evidence: | ChIP-PCR,ChIP | |
| Target gene experiment description: | We elucidated that both the chromatin conformational structure and histone modification change under hy_x0002_poxic conditions and enhance the expression of SLC2A3 based on the combined results of chromatin conformation capture (3C) and ChIP assays. KDM3A is recruited to the SLC2A3 locus in an HIF1-dependent manner and demethylates H3K9me2 so as to upregulate its expression.These findings provide novel insights into the interaction between HIF1 and KDM3A and also the epigenetic regulation of HIF1. |
About TF
| TF name : | HIF1A(HIF-1-alpha,HIF-1A,HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78) | |
| TF experiment: | ChIP | |
| TF experiment description: | Genome-wide analysis of HIF1 binding sites (chromatin immunoprecipitation[ChIP] with deep sequencing) of endothelial cells clarified that HIF1 mainly binds to the intergenic regions distal from tran_x0002_scriptional starting sites under both normoxia and hypoxia. |
About Function
| Enhancer function : | HIF1 is necessary to keep the close proximity of chromatin conformation at the kbp 35 regions of SLC2A3 loci |
| Enhancer function experiment: | ChIP-seq,3C-ChIP,ChIP-PCR |
| Enhancer function experiment description: |
This result indicated that HIF1 is necessary to keep the close proximity of chromatin conformation at the kbp 35 regions of SLC2A3 loci. Taking all of the ndings together, under normoxia, the kbp 35 region functions as an enhancer to induce the expression of SLC2A3 at a relatively low level and retains chromatin conformation also under normoxia. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
