About Enhancer
| Enhancer ID: | E_01_105 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr11:101981958-101982137   |
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| Biosample name: | HCT 116,SW620,SW480,RKO | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Colorectal Cancer | |
| DO: | DOID:9256 | |
| Mesh: | D015179 | |
| Distance from TSS: | Intron | |
| Pubmed ID: | 22337891 |
| Enhancer experiment: | ChIP,RT-PCR,Luciferase Reporter Assay | |
| Enhancer experiment description: | To test whether β-catenin/TCF4 complexes were responsible for enhancer activity, we mutated the consensus TCF motif to CGCTGAT.In transfected cells, levels of luciferase expressed from the YAP reporter were _x0001_60% of the lev_x0002_els observed with the YAP (WT) reporter.To test whether the residual activity of the YAP reporter was due to a second β-catenin binding site, cells were treated with the GSK3_x0001_inhibitor BIO. BIO treatment increased both pGL3-YAP (WT) and pGL3-YAP reporter activities suggesting that the YAP enhancer element contains a second, unidentified β-catenin binding site. |
About Target gene
| Target gene : | YAP1(COB1,YAP,YAP2,YAP65,YKI) | |
| Strong evidence: | -- | |
| Less strong evidence: | ChIP,RT-PCR,Luciferase Reporter Assay | |
| Target gene experiment description: | Because β-catenin is recruited to chromatin through interactions with TCF4, we next tested whether TCF4 bound to the YAP gene using ChIP assays.Here we demonstrate that β-catenin/TCF4 complexes bind a DNA enhancer element within the first intron of the YAP gene to drive YAP expression in CRC cells. As such, reducing β-catenin expression in CRC cells using shRNAs leads to decreased YAP mRNA and protein levels. |
About TF
| TF name : | TCF4(E2-2,FECD3,ITF-2,ITF2,PTHS,SEF-2,SEF2,SEF2-1,SEF2-1A,SEF2-1B,SEF2-1D,TCF-4,bHLHb19) | |
| TF experiment: | ChIP,Luciferase Reporter Assay | |
| TF experiment description: | To determine whether β-catenin/TCF4 bound a transcriptionally active YAP gene locus, we precipitated RNA polymerase II (RNAP) in ChIP assays and found that RNAP bound YAP. |
About Function
| Enhancer function : | β-Catenin/TCF4 complexes directly regulate YAP gene expression through a novel DNA Enhancer element. |
| Enhancer function experiment: | ChIP-seq,ChIP |
| Enhancer function experiment description: |
Here we demonstrate that β-Catenin/TCF4 complexes bind a DNA enhancer element within the first intron of the YAP gene to drive YAP expression in CRC cells. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
