About Enhancer
| Enhancer ID: | E_01_102 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr9:132491985-132492534   |
|
| Biosample name: | HFL-1 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Lung Cancer | |
| DO: | DOID:1324 | |
| Mesh: | D008175 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 22260630 |
| Enhancer experiment: | DNaseI-seq,ChIP,RT-PCR | |
| Enhancer experiment description: | We identified two DNase I-hypersensitive sites within the proximal promoter near the Egr-1 element and a novel distal site near − 8.6 kb. Functional analysis of the distal site revealed two elements that co-operate with basal promoter expression and a stimulus-dependent enhancer.. A specific binding site for C/EBPβ (CCAAT/enhancer-binding protein β) in the enhancer was directly responsible for inducible enhancer activity. |
About Target gene
| Target gene : | PTGES(MGST-IV,MGST1-L1,MGST1L1,MPGES,PGES,PIG12,PP102,PP1294,TP53I12,mPGES-1) | |
| Strong evidence: | -- | |
| Less strong evidence: | DNaseI-seq,ChIP,RT-PCR | |
| Target gene experiment description: | We therefore hypothesized that, apart from the proximal promoter harbouring a potentially important Egr-1 site, there must be other cis-acting regulatory elements required to recapitulate the full cytokine-dependent induction of mPGES-1 expression. Our results demonstrate the identification and characterization of a novel IL-1β (interleukin 1β)-responsive distal enhancer element controlled through C/EBPβ (CCAAT/enhancer-binding protein β) which contributes to the induction of endogenous mPGES-1. |
About TF
| TF name : | EGR1(AT225,G0S30,KROX-24,NGFI-A,TIS8,ZIF-268,ZNF225)CEBPB(C/EBP-beta,IL6DBP,NF-IL6,TCF5) | |
| TF experiment: | ChIP | |
| TF experiment description: | ChIP (chromatin immunoprecipitation) analysis demonstrated constitutive Egr-1 binding to the promoter and induced RNA polymerase II and C/EBPβ binding to the promoter and Enhancer respectively. |
About Function
| Enhancer function : | Interaction between Egr-1 and C/Eβ highlights the proximal promoter co-operation with a novel distal Enhancer element can regulate inducible mPGES-1 expression. |
| Enhancer function experiment: | ChIP,RT-PCR |
| Enhancer function experiment description: |
Knockout/knockdown studies established a functional role for C/Eβ in mPGES-1 gene regulation and the documented interaction between Egr-1 and C/Eβ highlights the proximal promoter co-operation with a novel distal Enhancer element in regulating inducible mPGES-1 expression. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
