About Enhancer
| Enhancer ID: | E_01_093 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr17:62028378-62028924   |
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| Biosample name: | HEK-293,HeLa | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 21711161 |
| Enhancer experiment: | Luciferase Reporter Assay | |
| Enhancer experiment description: | Upstream HS III sequences contained in a 574 bp fragment were previously shown to act as effective enhancers of minimal TKp activity in transfected human HEK293 and HeLa cells.To assess for enhancer--blocking activity, the same HS V fragments were inserted between the HS III enhancer and the downstream TKp and firefly luciferase (Luc) reporter gene. Significant decreases in enhancer activity were observed in both the forward and reverse orientations in both cell lines (p<0.05, n=5–6)(Fig. 2) |
About Target gene
| Target gene : | GH1(GH,GH-N,GHB5,GHN,IGHD1A,IGHD1B,IGHD2,hGH-N) | |
| Strong evidence: | -- | |
| Less strong evidence: | Luciferase Reporter Assay,Transfection | |
| Target gene experiment description: | Upstream HS III sequences contained in a 574 bp fragment were previously shown to act as effective enhancers of minimal TKp activity in transfected human HEK293 and HeLa cells.To assess for enhancer--blocking activity, the same HS V fragments were inserted between the HS III enhancer and the downstream TKp and firefly luciferase (Luc) reporter gene. Significant decreases in enhancer activity were observed in both the forward and reverse orientations in both cell lines (p<0.05, n=5–6)(Fig. 2) |
About TF
| TF name : | YY1(DELTA,GADEVS,INO80S,NF-E1,UCRBP,YIN-YANG-1)CTCF(MRD21) | |
| TF experiment: | ChIP | |
| TF experiment description: | In terms of enhancer-blocking activity, the decrease in either CTCF or Yy1 was associated with a significant *50% increase or rescue of enhancer activity in the case of HSVR, and*80% when siRNA for both CTCF and Yy1 were used. |
About Function
| Enhancer function : | HS V sequences contain efficient Enhancer-blocking activity |
| Enhancer function experiment: | Luciferase Reporter Assay |
| Enhancer function experiment description: |
To assess for enhancerblocking activity, the same HS V fragments were inserted between the HS III enhancer and the downstream TKp and firefly luciferase (Luc) reporter gene |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
