About Enhancer
| Enhancer ID: | E_01_092 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr12:7940709-7941994   |
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| Biosample name: | HEK-293 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | <2KB | |
| Pubmed ID: | 21689639 |
| Enhancer experiment: | Luciferase Reporter Assay | |
| Enhancer experiment description: | Genome sequence analysis of the enhancer region of the human NANOG gene showed the presence of two conserved Tcf/Lef--binding motifs: CAAAGA at positions --1206 to --1190 (designated as Lef #1) and an inverted GTTTCA sequence at ?1020 to ?1004 (designated as Lef #2) (Fig. 1A). To investigate whether Tcf/Lef elements located in the enhancer region play a role in the regulation of NANOG gene transcription, we isolated a 1286--bp fragment of the enhancer region of the human NANOG gene and subcloned it into a firefly luciferase reporter vector, yielding pNanog--Luc(--1286/--1). |
About Target gene
| Target gene : | NANOG(NANOG) | |
| Strong evidence: | -- | |
| Less strong evidence: | Luciferase Reporter Assay | |
| Target gene experiment description: | To investigate whether Tcf/Lef elements located in the enhancer region play a role in the regulation of NANOG gene transcription, we isolated a 1286--bp fragment of the enhancer region of the human NANOG gene and subcloned it into a firefly luciferase reporter vector, yielding pNanog--Luc(--1286/--1). |
About TF
| TF name : | LEF1(LEF-1,TCF10,TCF1ALPHA,TCF7L3) | |
| TF experiment: | ChIP | |
| TF experiment description: | ChIP experiments demonstrated that b-catenin and Lef1 bind to the Enhancer region of the NANOG gene. Our present findings would appear to indicate that the Tcf/Lef element in the Enhancer region of the Human NANOG gene functions in transcriptional activation of the Human NANOG gene. |
About Function
| Enhancer function : | -- |
| Enhancer function experiment: | -- |
| Enhancer function experiment description: |
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About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
