Enhancer ID: | E_01_077 | |
Enhancer symbol: | -- | |
Species: | Human | |
Position : | chr8:128747315-128748315 |
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Biosample name: | HCT 116 | |
Experiment class : | Low+High throughput |
Enhancer type: | Enhancer | |
Disease: | Colorectal Cancer | |
DO: | DOID:9256 | |
Mesh: | D015179 | |
Distance from TSS: | <2KB | |
Pubmed ID: | 21533051 |
Enhancer experiment: | ChIP,PCR,ChIP-seq | |
Enhancer experiment description: | A diagram of novel ß-catenin binding regions (labeled A through F) and characterized Wnt responsive enhancers (labeled 5′ WRE and 3′ WRE) that were identified in the screen.Real-time PCR analysis of DNA elements precipitated using ß-catenin specific antibodies in ChIP assays conducted in HCT116 (black bars) or HEK293 (gray bars). |
Target gene : | MYC(MRTLC,bHLHe39,c-Myc) | |
Strong evidence: | q3C | |
Less strong evidence: | -- | |
Target gene experiment description: | Using quantitative chromatin conformation capture, I show that the distal WREs are aligned with the MYC promoter through large chromatin loops. |
TF name : | TCF4(E2-2,FECD3,ITF-2,ITF2,PTHS,SEF-2,SEF2,SEF2-1,SEF2-1A,SEF2-1B,SEF2-1D,TCF-4,bHLHb19) | |
TF experiment: | ChIP-seq | |
TF experiment description: | In the present study, I characterize five novel β-catenin/TCF4 binding sites that were identified using a ChIP-Seq screen for β-catenin binding regions in the human HCT116 colon cancer cell line. |
Enhancer function : | The architecture of the MYC promoter is comprised of distal elements. |
Enhancer function experiment: | 3C |
Enhancer function experiment description: |
These findings suggest that the architecture of the MYC promoter is comprised of distal elements that are juxtaposed through large chromatin loops and that -catenin/TCF4 complexes utilize this conformation to activate MYCexpression in colon cancer cells. |
SNP ID: | rs6983267 | |
SNP position: | 128413305 |
SNP experiment: | qRT-PCR,3C |
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