Enhancer ID: | E_01_061 | |
Enhancer symbol: | -- | |
Species: | Human | |
Position : | chr5:158755252-158761634 |
|
Biosample name: | HeLa,THP-1 | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 21402921 |
Enhancer experiment: | ChIP-qPCR,ChIP | |
Enhancer experiment description: | To test this hypothesis, we chose the top 10 genes specifically induced by TNF-α in THP-1 cells, but not HeLa cells, from microarray data (Fig. 1B), and verified five of them (CCL3, CCL4, IL12B, C3AR1, and GPR84) had PU.1 peaks as well as THP-1–specific p65 binding sites within 30 kb of their promoters (Fig. 4A). |
Target gene : | IL12B(CLMF,CLMF2,IL-12B,IMD28,IMD29,NKSF,NKSF2) | |
Strong evidence: | -- | |
Less strong evidence: | ChIP-chip,RT-qPCR,ChIP-qPCR | |
Target gene experiment description: | To test this hypothesis, we chose the top 10 genes specifically induced by TNF-α in THP-1 cells, but not HeLa cells, from microarray data (Fig. 1B), and verified five of them (CCL3, CCL4, IL12B, C3AR1, and GPR84) had PU.1 peaks as well as THP-1–specific p65 binding sites within 30 kb of their promoters (Fig. 4A). |
TF name : | SPI1(OF,PU.1,SFPI1,SPI-1,SPI-A)CEBPB(C/EBP-beta,IL6DBP,NF-IL6,TCF5)NFKB1(CVID12,EBP-1,KBF1,NF-kB,NF-kB1,NF-kappa-B1,NF-kappaB,NFKB-p105,NFKB-p50,NFkappaB,p105,p50) | |
TF experiment: | ChIP-qPCR,ChIP | |
TF experiment description: | Strikingly, we found that all these genes gained responsiveness to TNF-α stimulation only when both PU.1 and C/EBPα were coexpressed in HeLa cells (Fig. 4B). To test the chromatin events near these genes, we further identified eight THP-1–specific p65 locations (P1–P8; Fig. 4A) around these five genes and performed ChIP/quantitative PCR (qPCR) to detect p75 occupancy and active chromatin. |
Enhancer function : | The diversity of transcriptional programs in mammalian cells arises, at least in part,is from preexisting enhancers that are established by cell-specific transcription factors. |
Enhancer function experiment: | ChIP |
Enhancer function experiment description: |
These results strongly support the model that combinatorial binding of THP-1–specific TFs PU.1 and C/EBPα synergistically create a distinct group of enhancers and mediate selection of a subset of monocyte/macrophage-specific NF-κB target genes for specific induction by allowing the binding of p65 to these locations for productive gene expression. |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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