About Enhancer

Enhancer ID: E_01_061
Enhancer symbol: --
Species: Human
Position : chr5:158755252-158761634
Biosample name: HeLa,THP-1
Experiment class : Low throughput
Enhancer type: Enhancer
Disease: --
DO: --
Mesh: --
Distance from TSS: >2KB
Pubmed ID:  21402921
Enhancer experiment: ChIP-qPCR,ChIP
Enhancer experiment description: To test this hypothesis, we chose the top 10 genes specifically induced by TNF-α in THP-1 cells, but not HeLa cells, from microarray data (Fig. 1B), and verified five of them (CCL3, CCL4, IL12B, C3AR1, and GPR84) had PU.1 peaks as well as THP-1–specific p65 binding sites within 30 kb of their promoters (Fig. 4A).

About Target gene

Target gene : IL12B(CLMF,CLMF2,IL-12B,IMD28,IMD29,NKSF,NKSF2)
Strong evidence: --
Less strong evidence: ChIP-chip,RT-qPCR,ChIP-qPCR
Target gene experiment description: To test this hypothesis, we chose the top 10 genes specifically induced by TNF-α in THP-1 cells, but not HeLa cells, from microarray data (Fig. 1B), and verified five of them (CCL3, CCL4, IL12B, C3AR1, and GPR84) had PU.1 peaks as well as THP-1–specific p65 binding sites within 30 kb of their promoters (Fig. 4A).

About TF

TF name : SPI1(OF,PU.1,SFPI1,SPI-1,SPI-A)CEBPB(C/EBP-beta,IL6DBP,NF-IL6,TCF5)NFKB1(CVID12,EBP-1,KBF1,NF-kB,NF-kB1,NF-kappa-B1,NF-kappaB,NFKB-p105,NFKB-p50,NFkappaB,p105,p50)
TF experiment: ChIP-qPCR,ChIP
TF experiment description: Strikingly, we found that all these genes gained responsiveness to TNF-α stimulation only when both PU.1 and C/EBPα were coexpressed in HeLa cells (Fig. 4B). To test the chromatin events near these genes, we further identified eight THP-1–specific p65 locations (P1–P8; Fig. 4A) around these five genes and performed ChIP/quantitative PCR (qPCR) to detect p75 occupancy and active chromatin.

About Function

Enhancer function : The diversity of transcriptional programs in mammalian cells arises, at least in part,is from preexisting enhancers that are established by cell-specific transcription factors.
Enhancer function experiment: ChIP
Enhancer function
experiment description:
These results strongly support the model that combinatorial binding of THP-1–specific TFs PU.1 and C/EBPα synergistically create a distinct group of enhancers and mediate selection of a subset of monocyte/macrophage-specific NF-κB target genes for specific induction by allowing the binding of p65 to these locations for productive gene expression.

About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs