Enhancer ID: | E_01_060 | |
Enhancer symbol: | -- | |
Species: | Human | |
Position : | chr2:113597789-113600339 |
|
Biosample name: | HeLa,THP-1 | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | -- | |
DO: | -- | |
Mesh: | -- | |
Distance from TSS: | >2KB | |
Pubmed ID: | 21402921 |
Enhancer experiment: | ChIP-qPCR,ChIP | |
Enhancer experiment description: | Premarked enhancers and promoters predict cell-specific p65 binding.(Fig.2) |
Target gene : | IL1B(IL-1,IL1-BETA,IL1F2,IL1beta) | |
Strong evidence: | -- | |
Less strong evidence: | ChIP-qPCR,ChIP | |
Target gene experiment description: | Premarked enhancers and promoters predict cell-specific p65 binding.(Fig.2) |
TF name : | SPI1(OF,PU.1,SFPI1,SPI-1,SPI-A)CEBPB(C/EBP-beta,IL6DBP,NF-IL6,TCF5)NFKB1(CVID12,EBP-1,KBF1,NF-kB,NF-kB1,NF-kappa-B1,NF-kappaB,NFKB-p105,NFKB-p50,NFkappaB,p105,p50) | |
TF experiment: | ChIP-qPCR,ChIP | |
TF experiment description: | Strikingly, we found that all these genes gained responsiveness to TNF-α stimulation only when both PU.1 and C/EBPα were coexpressed in HeLa cells (Fig. 4B). To test the chromatin events near these genes, we further identified eight THP-1–specific p65 locations (P1–P8; Fig. 4A) around these five genes and performed ChIP/quantitative PCR (qPCR) to detect p75 occupancy and active chromatin. |
Enhancer function : | The diversity of transcriptional programs in mammalian cells arises, at least in part,is from preexisting enhancers that are established by cell-specific transcription factors. |
Enhancer function experiment: | ChIP |
Enhancer function experiment description: |
These results strongly support the model that combinatorial binding of THP-1–specific TFs PU.1 and C/EBPα synergistically create a distinct group of enhancers and mediate selection of a subset of monocyte/macrophage-specific NF-κB target genes for specific induction by allowing the binding of p65 to these locations for productive gene expression. |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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