About Enhancer
| Enhancer ID: | E_01_044 | |
| Enhancer symbol: | androgen Enhancer | |
| Species: | Human | |
| Position : | chr19:50850114-50850982   |
|
| Biosample name: | LNCaP | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | Prostate Cancer | |
| DO: | DOID:10283 | |
| Mesh: | D011471 | |
| Distance from TSS: | >2KB | |
| Pubmed ID: | 21051589 |
| Enhancer experiment: | Luciferase Reporter Assay,RT-PCR | |
| Enhancer experiment description: | The luciferase activity of the pPSABHE reporter vector, which contains the promoter (2589 to 241) and androgen enhancer regions (24801 to 23933) of the PSA gene, increased by 8--fold after IL--6 treatment. |
About Target gene
| Target gene : | KLK3(APS,KLK2A1,PSA,hK3) | |
| Strong evidence: | -- | |
| Less strong evidence: | Luciferase Reporter Assay | |
| Target gene experiment description: | The luciferase activity of the pPSABHE reporter vector, which contains the promoter (2589 to 241) and androgen enhancer regions (24801 to 23933) of the PSA gene, increased by 8--fold after IL--6 treatment. |
About TF
| TF name : | STAT3(ADMIO,ADMIO1,APRF,HIES) | |
| TF experiment: | Luciferase Reporter Assay | |
| TF experiment description: | Transient gene expression assays of cells cotransfected with the pPSABHE reporter vector and the STAT3 expression vector revealed that transient overexpression of STAT3 significantly upregulated the luciferase activity of the pPSABHE reporter vector in an androgen-independent manner. |
About Function
| Enhancer function : | Results from 5'-deletion reporter assays revealed that the effects of IL-6 appear to be mediated via an androgen Enhancer region (24801 to 23933), which is dependent on the signal transducer and activator of the transcription 3 (STAT3) pathway. |
| Enhancer function experiment: | Luciferase Reporter Assay |
| Enhancer function experiment description: |
Moreover, we also found that IL-6 treatment did not further enhance the stimulatory effect of transient overexpression of STAT3 on luciferase activity of the pPSABHE reporter vector, suggesting that the STAT3 signaling pathway, which is dependent on the Enhancer region of PSA gene, is the major pathway involved in the IL-6–regulated expression of the PSA gene in LNCaP cells. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
