About Enhancer
| Enhancer ID: | E_01_031 | |
| Enhancer symbol: | MnSOD Enhancer | |
| Species: | Human | |
| Position : | chr6:160101620-160102231   |
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| Biosample name: | PC-3 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | Intron | |
| Pubmed ID: | 20061391 |
| Enhancer experiment: | RT-PCR,Luciferase Reporter Assay,EMSA,ChIP | |
| Enhancer experiment description: | For reporter gene activity, a luciferase assay was performed by co-transfecting the cells with the p53 expression vector and vector containing the enhancer (I2E) and the promoter (−3400/+24, −555/+24, or −210/+24) of the human MnSOD gene in the pGL3 reporter vector. |
About Target gene
| Target gene : | SOD2(IPO-B,IPOB,MNSOD,MVCD6,Mn-SOD) | |
| Strong evidence: | -- | |
| Less strong evidence: | Luciferase Reporter Assay,EMSA,PCR,ChIP | |
| Target gene experiment description: | These results suggest that the positive effect of p53 on MnSOD expression is dependent on the presence of the intronic enhancer element of the MnSOD gene.FIGURE 3 |
About TF
| TF name : | TP53(BCC7,BMFS5,LFS1,P53,TRP53) | |
| TF experiment: | PCR,EMSA,ChIP | |
| TF experiment description: | The promoter fragment amplified after p53 overexpression was decreased when the ChIP was preformed with the Sp1 antibody.These results are consistent with the DNA-binding data obtained by EMSA, suggesting that p65 binding to the MnSOD enhancer may contribute to the induction of MnSOD gene transcription. |
About Function
| Enhancer function : | The positive effect of p53 on MnSOD expression is dependent on the presence of the intronic Enhancer element of the MnSOD gene |
| Enhancer function experiment: | Luciferase Reporter Assay |
| Enhancer function experiment description: |
Addition of the Enhancer element to the MnSOD promoter construct led to the bi-directional effect of p53 as observed with the endogenous MnSOD gene.These results suggest that the positive effect of p53 on MnSOD expression is dependent on the presence of the intronic Enhancer element of the MnSOD gene. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
