About Enhancer

Enhancer ID: E_01_025
Enhancer symbol: CD200 distal Enhancer
Species: Human
Position : chr3:112045517-112046117
Biosample name: HUVEC
Experiment class : Low throughput
Enhancer type: Enhancer
Disease: Graft Rejection,Autoimmune Diseases,Spontaneous Abortion
DO: --
Mesh: D006084,D001327,D000022
Distance from TSS: >2KB
Pubmed ID:  19386363
Enhancer experiment: Luciferase Reporter Assay,ChIP,PCR,DNase I Hypersensitivity Assay
Enhancer experiment description: Using a DNase I hypersensitivity assay, we identified a distal enhancer region responsible for inducible expression of CD200.Generation of a luciferase reporter construct containing both CD200 core promoter and potential enhancer region

About Target gene

Target gene : CD200(MOX1,MOX2,MRC,OX-2)
Strong evidence: --
Less strong evidence: Site-Directed Mutagenesis
Target gene experiment description: The potential regulatory region (−5677/−5077) enhanced CD200 promoter activity after stimulation of IFN- and TNF-˛ but this function was abolished without C/EBPˇ binding site in the core promoter Site-directed mutagenesis was performed to generate four mutant constructs

About TF

TF name : NFKB1(CVID12,EBP-1,KBF1,NF-kB,NF-kB1,NF-kappa-B1,NF-kappaB,NFKB-p105,NFKB-p50,NFkappaB,p105,p50)STAT1(CANDF7,IMD31A,IMD31B,IMD31C,ISGF-3,STAT91)IRF1(IRF-1,MAR)
TF experiment: qPCR,ChIP
TF experiment description: The results of ChIP and PCR suggest that NF-kB, STAT1 and IRF-1 interact with the Enhancer region containing the corresponding binding sites following stimulation with IFN-r_x0002_and TNF-a.

About Function

Enhancer function : All the above findings suggest that IFN-γ and TNF-α induce CD200 expression through a 5 upstream Enhancer and that NF-κB, STAT1 and IRF-1 play pivotal roles in this process.
Enhancer function experiment: ChIP
Enhancer function
experiment description:
These results suggest that NF-kB, STAT1 and IRF-1 interact with the Enhancer region containing the corresponding binding sites following stimulation with IFN-γ and TNF-α.

About SNP

SNP ID: --
SNP position: --
SNP experiment: --

Enhancer associated network

The number on yellow line represents the distance between enhancer and target gene

Expression of target genes for the enhancer


Enhancer associated SNPs