Enhancer ID: | E_01_025 | |
Enhancer symbol: | CD200 distal Enhancer | |
Species: | Human | |
Position : | chr3:112045517-112046117 |
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Biosample name: | HUVEC | |
Experiment class : | Low throughput |
Enhancer type: | Enhancer | |
Disease: | Graft Rejection,Autoimmune Diseases,Spontaneous Abortion | |
DO: | -- | |
Mesh: | D006084,D001327,D000022 | |
Distance from TSS: | >2KB | |
Pubmed ID: | 19386363 |
Enhancer experiment: | Luciferase Reporter Assay,ChIP,PCR,DNase I Hypersensitivity Assay | |
Enhancer experiment description: | Using a DNase I hypersensitivity assay, we identified a distal enhancer region responsible for inducible expression of CD200.Generation of a luciferase reporter construct containing both CD200 core promoter and potential enhancer region |
Target gene : | CD200(MOX1,MOX2,MRC,OX-2) | |
Strong evidence: | -- | |
Less strong evidence: | Site-Directed Mutagenesis | |
Target gene experiment description: | The potential regulatory region (−5677/−5077) enhanced CD200 promoter activity after stimulation of IFN- and TNF-˛ but this function was abolished without C/EBPˇ binding site in the core promoter Site-directed mutagenesis was performed to generate four mutant constructs |
TF name : | NFKB1(CVID12,EBP-1,KBF1,NF-kB,NF-kB1,NF-kappa-B1,NF-kappaB,NFKB-p105,NFKB-p50,NFkappaB,p105,p50)STAT1(CANDF7,IMD31A,IMD31B,IMD31C,ISGF-3,STAT91)IRF1(IRF-1,MAR) | |
TF experiment: | qPCR,ChIP | |
TF experiment description: | The results of ChIP and PCR suggest that NF-kB, STAT1 and IRF-1 interact with the Enhancer region containing the corresponding binding sites following stimulation with IFN-r_x0002_and TNF-a. |
Enhancer function : | All the above findings suggest that IFN-γ and TNF-α induce CD200 expression through a 5 upstream Enhancer and that NF-κB, STAT1 and IRF-1 play pivotal roles in this process. |
Enhancer function experiment: | ChIP |
Enhancer function experiment description: |
These results suggest that NF-kB, STAT1 and IRF-1 interact with the Enhancer region containing the corresponding binding sites following stimulation with IFN-γ and TNF-α. |
SNP ID: | -- | |
SNP position: | -- |
SNP experiment: | -- |
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