About Enhancer
| Enhancer ID: | E_01_020 | |
| Enhancer symbol: | -- | |
| Species: | Human | |
| Position : | chr7:117228051-117229440   |
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| Biosample name: | Caco-2 | |
| Experiment class : | Low throughput |
| Enhancer type: | Enhancer | |
| Disease: | -- | |
| DO: | -- | |
| Mesh: | -- | |
| Distance from TSS: | Intron | |
| Pubmed ID: | 26673704 |
| Enhancer experiment: | RT-qPCR,4C,ChIP,CRISPR/Cas9 | |
| Enhancer experiment description: | Two pairs of gRNAs flanking the −20.9 kb CTCF site and the intron 11 enhancer core were identifed using the CRISPR Design Program |
About Target gene
| Target gene : | CFTR(ABC35,ABCC7,CF/MRP,MRP7,TNR-CFTR,dJ760C5.1\) | |
| Strong evidence: | CRISPR/Cas9 | |
| Less strong evidence: | -- | |
| Target gene experiment description: | Here we use a combination of CRISPR/Cas9 editing of cis-regulatory elements and siRNA-mediated depletion of architectural proteins to determine the relative contribution of structural elements and enhancers to the higher order structure and expression of the CFTR locus. |
About TF
| TF name : | FOXA1(HNF3A,TCF3A)FOXA2(HNF3B,TCF3B)HNF1A(HNF-1A,HNF1,HNF4A,IDDM20,LFB1,MODY3,TCF-1,TCF1)CDX2(CDX-3/AS,CDX3,CDX2) | |
| TF experiment: | 4C,ChIP,CRISPR/Cas9 | |
| TF experiment description: | Approximately 1.2 encompass_x0002_ing the binding sites for critical transcription factors driv_x0002_ing the intron 11 Enhancer (FOXA1/2,HNF1 and CDX2). |
About Function
| Enhancer function : | Deletion of a strong enhancer in intron 11 has little impact on the 3D architecture, but represses CFTR gene expression. |
| Enhancer function experiment: | CRISPR/Cas9,RT-qPCR |
| Enhancer function experiment description: |
Approximately 1.2 kb encompassing the binding sites for critical transcription factors driving the intron 11 enhancer (FOXA1/2, HNF1 and CDX2).The del11 clones show a significant reduction in RNAP II occupancy at the promoter, intron 1, 10 and 11, suggesting a global loss of the polymerase across the CFTR locus.The intron 11 enhancer participates in recruitment of RNAPII to the CFTR promoter as well as to intronic regions. |
About SNP
| SNP ID: | -- | |
| SNP position: | -- |
| SNP experiment: | -- |
Enhancer associated network
The number on yellow line represents the distance between enhancer and target gene
