External factors
| Category: |
Chemical compounds |
| Experiment: |
SA-β-gal activity assay//Western blot |
| Description: |
70% of HCT-116 cells showed increased SA-β-gal activity after exposure to CNF1 for 72 h, whereas less than 9% of untreated and polyploid cell-derived daughter cells were positive for SA-β-gal activity.To confirm CNF1-induced senescent arrest, we analysed the expression of cell-cycle checkpoints proteins and observed that the expression of p53, p21 and p16 in HCT-116 cells were significantly increased after 24 h exposure to CNF1, and further increased after prolonged exposure during 72 h as compared with untreated control cells. |
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Regulatory relationship
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| Regulatory pathway:
|
p53//p21//p16 |
| R-EF-Pathway:
|
Upregulation//Upregulation//Upregulation |
| Official symbol(s): |
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TP53//CDKN1A//
|
| Pathway experiment: |
Western blot |
| Pathway description: |
To confirm CNF1-induced senescent arrest, we analysed the expression of cell-cycle checkpoints proteins and observed that the expression of p53, p21 and p16 in HCT-116 cells were significantly increased after 24 h exposure to CNF1, and further increased after prolonged exposure during 72 h as compared with untreated control cells. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Pathway view
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