External factors
| External factors: |
Sargahydroquinoic acid |
| Category: |
Chemical compounds |
| Experiment: |
Western blot//SA-β-gal activity assay//Cell viability assay |
| Description: |
Short-term exposure to low doseH2O2 elevated protein expression of three senescence biomarkers, p53, p21, and p16, whose levels were suppressed by SHQA in a dose-dependentmanner.Whereas,SHQA suppressed the SA-β-Gal positive cell abundance at all three tested concentrations. The SA-β-Gal positive cell percentages were around 24%, 18% and 12% when pretreated with 0.5, 1.0, or 2.0 μM SHQA, respectively. Culturing and passaging HUVECs in growth medium supplemented with 2.0 μM SHQA remarkably delayed the cellular growth arrest. When cultured in normal medium supplemented with DMSO, the growth of HUVECs was arrested at 23.9 PDL (population doubling), whereas the supplementation of SHQA extended the cell growth to 26.3 PDL.The SA-β-Gal positive cells were significantly lower in cells supplemented with SHQA at PDL24 (24%), compared to cells cultured with DMSO at PDL23 (51%). SHQA treatment also reduced the protein expression levels of p53, p21, and p16 in senescent cells. |
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
383
Warning: Undefined variable $target_genes in
/data/html/Aging-ReG/public/browse_detail_2.php on line
385
Warning: Trying to access array offset on null in
/data/html/Aging-ReG/public/browse_detail_2.php on line
383
Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Official symbol(s): |
AKT//MTOR |
| Target gene experiment: |
Western blot |
| Target gene description: |
However, the H2O2-induced hyperphosphorylation of Akt and mTOR was suppressed by SHQA at 1.0 μM and 2.0 μM.SHQA inhibited the phosphoryla tion of Akt, mTOR at all three concentrations tested. |
| Regulatory pathway:
|
Akt-mTOR |
| R-EF-Pathway:
|
Downregulation |
| Official symbol(s): |
Warning: Undefined variable $name_all in /data/html/Aging-ReG/public/browse_detail_2.php on line 525
AKT1-MTOR
|
| Pathway experiment: |
Western blot |
| Pathway description: |
However, the H2O2-induced hyperphosphorylation of Akt and mTOR was suppressed by SHQA at 1.0 μM and 2.0 μM.SHQA inhibited the phosphoryla tion of Akt, mTOR at all three concentrations tested. |
Aging network
Warning: Undefined array key 1 in
/data/html/Aging-ReG/public/browse_detail_2.php on line
578
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.