External factors
| External factors: |
SRT1720 |
| Category: |
Chemical compounds |
| Cell name: |
Dermal fibroblasts |
| Experiment: |
SA-β-gal activity assay//Western blot//Confocal microscopy |
| Description: |
After treatment with SRT1720, cellular senescence was examined by SA-β-gal staining (senescent cells were stained blue) and examined by bright-field microscopy (original magnification ×200). Scale bar, 50 μM. Percentages of blue cells per 50 cells were calculated. HDFs were also exposed to SRT1720 (10 μM) for 1 h and then protein levels were analyzed by immunoblotting. |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation |
| Official symbol(s): |
SIRT1 |
| Target gene experiment: |
Western blot |
| Target gene description: |
HDFs were exposed to SRT1720 (10 μM) for 1 h and then protein levels were analyzed by immunoblotting. |
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| Regulatory pathway:
|
SIRT1-NF-κB |
| R-EF-Pathway:
|
Upregulation |
| Pathway experiment: |
Western blot |
| Pathway description: |
HDFs were subjected to immunoprecipitation analysis using SIRT1 antibody or control IgG, and then blotted with antibodies-recognizing ac-NF-κB, LC3, and Beclin-1. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
Pathway view
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