External factors
| External factors: |
Apremilast |
| Category: |
Chemical compounds |
| Phenotype: |
Osteoarthritis |
| Experiment: |
SA-β-gal activity assay |
| Description: |
IL-17 stimulation induced a 3.7-fold increase of SA-β-gal activity, while 1 ?M apremilast reduced its activity to only 2.1-fold |
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Regulatory relationship
| R-EF-Target gene:
|
Upregulation |
| Official symbol(s): |
SIRT1 |
| Target gene experiment: |
RT-qPCR//Western blot |
| Target gene description: |
Compared to the non-treated cells, IL-17 reduced the mRNA level of SIRT1 to 53%, which was recovered to 92%, close to the baseline, in the presence of 1 ?M apremilast. It was also confirmed that apremilast rescued the protein level of SIRT1 which had been significantly reduced by IL-17. As revealed in Fig. 7A, in normal SIRT-expressing cells, apremilast significantly suppressed cellular senescence, however, its effect was almost abolished in SIRT1-silenced cells. The results from western blot analysis in Fig. 7B and C revealed that the inhibitory effect of apremilast on PAI-1 and p21 was abolished by the silencing of SIRT1. |
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Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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