External factors
| External factors: |
Size of MSCs |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//Western blot |
| Description: |
SA β-gal-positive cells were considerably greater in both groups by P13. However, SA β-gal activity in small cells (8 ± 3%) was significantly lower than that in control cells (25 ± 6%). In both groups, cells became flattened and enlarged, especially the control cells, most of which exhibited these morphological changes. In parallel, Western blot analysis revealed enhanced expression of p16, p21, phosphorylated p38 (P-p38), and phosphorylated p53 (P-p53) in control cells when compared to small cells at P13. |
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Regulatory relationship
| R-EF-Target gene:
|
Downregulation |
| Official symbol(s): |
GROA//CXCL8 |
| Target gene experiment: |
Cytokine array//ELISA |
| Target gene description: |
Notably, both GROa and IL-8 secretion were significantly low in small cells. The secretion of GROa or IL-8 gradually increased with subsequent passages in both groups. Interestingly, cells of the two groups could be distinguished based on the concentration and rate of increase in GROa and IL-8 secretion with passaging. The increase in SASP-related secretion (GROa and IL-8) with cell passaging was markedly lower in small cells. |
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Aging network
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Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.
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