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Aging Overview

External factors

External factors: ALA
Aging type: Accelerate
Aging characteristic:
Category: Chemical compounds
Phenotype: Breast cancer
Experimental category: L
Tissue type: --
Cell name: MDA-MB-231 cancer cells
PMID: 33321563
Experiment: Western blot//PCR
Description: Cytosolic expression of HMGB1 was increased in the ALA and ALA+RT groups, whereas nuclear expression levels of HMGB1 were decreased in these groups compared to the control. Moreover, expression levels of p53 and activation of p38 mitogen-activated protein kinase (MAPK) and NF- κB were significantly increased in the ALA+RT group compared to the control. The expres- sion levels of IL-6, IL-8, and Cxcl1 were measured by qPCR to additionally examine the effect of ALA on cellular senes - cence in irradiated cells. Increased levels of the correspond- ing mRNAs were observed in the ALA+RT group compared to all groups. The expression of p21, a cyclin- dependent kinase (Cdk) inhibitor that induces senescence through cell cycle arrest, was significantly increased in the ALA+RT group compared to the other groups.



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Regulatory relationship

Target gene: HMGB1
R-EF-Target gene: Downregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation//Upregulation
Official symbol(s): HMGB1
Target gene experiment: Western blot
Target gene description: Cytosolic expression of HMGB1 was increased in the ALA and ALA+RT groups, whereas nuclear expression levels of HMGB1 were decreased in these groups compared to the control.


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Regulatory pathway: --
R-EF-Pathway: --
Pathway experiment: --
Pathway description: --


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.




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