External factors
| Category: |
Chemical compounds |
| Experimental category: |
HL |
| Experiment: |
SA-β-gal activity assay//RT-qPCR//Western blot |
| Description: |
tBHP treated and control cells were stained for SA-β-galactosidase activity on D9 of the experiment. RT-qPCR was performed to check for p21 mRNA levels. Protein expression of phosphorylated pRB, p53, p-p53 (Serin15) and p21 was measured by Westernblot on D4 and D9 of the experiment. |
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Regulatory relationship
| R-EF-Pathway:
|
Upregulation |
| Official symbol(s): |
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TP53
|
| Pathway experiment: |
Western blot |
| Pathway description: |
Protein expression of phosphorylated pRB, p53, p-p53 (Serin15) and p21 was measured by Westernblot on D4 and D9 of the experiment. |
Aging network
Annotation:
The green line represents Upregulation.
The purple line represents Downregulation.
The orange line represents Activation.
The yellow line represents Inhibition.
The gray line represents Unclear.